Effect of Steroids on Interleukin-1beta and Tumor Necrosis Factor-alpha Expression due to High Tidal Volume in the Rat Ventilator-induced Lung Injury Model.
- Author:
Byung Hak SO
1
;
Dong Rul OH
;
Mi Jin LEE
;
Won Jae LEE
;
Se Kyung KIM
;
Young Pil WANG
;
Suk Joo RHA
Author Information
1. Department of Emergency Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. emodr@mdhouse.com
- Publication Type:Original Article
- Keywords:
Ventilator-induced lung injury;
Interleukin-1beta;
Tumor necrosis factor-alpha;
Methylprednisolone
- MeSH:
Animals;
Humans;
Immune Sera;
Interleukin-1beta*;
Interleukins;
Lung;
Male;
Methylprednisolone;
Models, Animal;
Oxygen;
Rats*;
Rats, Sprague-Dawley;
Respiration, Artificial;
Steroids*;
Stress, Mechanical;
Tidal Volume*;
Tumor Necrosis Factor-alpha*;
Ventilation;
Ventilator-Induced Lung Injury*
- From:Journal of the Korean Society of Emergency Medicine
2002;13(2):141-146
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Although mechanical ventilation is used to improve oxygenation, this strategy can impose injurious mechanical stress on lung tissue. This is recognized as ventilator-induced lung injury (VILI). Recently, several studies have reported that inflammatory cells and mediators play an important role in the progression of VILI. This study was designed to investigate, in a rat model, the expression of interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha, as well as the anti-inflammatory activity of steroids in VILI. METHODS: Male Sprague-Dawley rats were divided into 3 groups. The control group received low tidal volume (6ml/kg) ventilation. The second group received high tidal volume (25 ml/kg) ventilation and the third group received high tidal volume (25 ml/kg) ventilation with methylprednisolone (30 mg/kg) treatment. After 60 minutes of mechanical ventilation, the animals in each group were sacrificed. Resected lungs were immunostained with antisera for IL-1beta and TNF-alpha. The stained areas were evaluated with an image analyzer. RESULTS: In the groups that received a high tidal volume, the number of positive pixels in IL-1 beta and TNF-alpha was significantly higher than it was in the low tidal volume group. The high tidal volume group showed greater expression of I L - 1beta and TNF-alpha,but this was significantly decreased by methylprednisolone. CONCLUSION: Our study suggests that an inflammatory response related with IL-1beta and TNF-alpha is involved in the development of VILI. The expression of IL-1beta and TNF-alpha was lessened by treatment with methylprednisolone, which might have contributed to improving lung dysfunction after mechanical ventilation.
