TNF-α Inhibitor Reduces Odontoclast Formation in Diabetes Rats with Ligature-Induced Periodontitis.
10.11620/IJOB.2017.42.3.137
- Author:
Ji Hye KIM
1
;
Ae Ri KIM
;
Yun Hui CHOI
;
Dong Eun LEE
;
Gye Hyeong WOO
;
Eun Jung BAK
;
Yun Jung YOO
Author Information
1. Department of Dental Hygiene, Jeonju kijeon College, Jeonju, Republic of Korea.
- Publication Type:Original Article
- Keywords:
TNF-α;
odontoclasts;
diabetes;
periodontiti
- MeSH:
Acid Phosphatase;
Administration, Intravenous;
Animals;
Cathepsin K;
Cathepsins;
Immunohistochemistry;
Infliximab;
Ligation;
Molar;
Necrosis;
Osteoclasts*;
Periodontitis*;
Rats*;
Streptozocin
- From:International Journal of Oral Biology
2017;42(3):137-142
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To determine the effect of the tumor necrosis factor-α (TNF-α) in odontoclast formation, we administrated a TNF-α inhibitor in rats with diabetes rats with periodontitis. The rats included in the study were divided into three groups: control rats without diabetes or periodontitis (the C group), rats with periodontitis and diabetes (the PD group), and rats with periodontitis and diabetes treated by infliximab, the TNF inhibitor (the PD+infliximab group). The PD and PD+ infliximab groups received intravenous administrations of streptozotocin (STZ, 50 mg/kg) to induce diabetes. After 7 days of STZ injections, the mandibular first molars were ligatured to induce periodontitis. The PD+infliximab group was intrapenitoneally administrated by infliximab (5 mg/kg). On days 3 and 20 after the ligature administration, odontoclast formation along root surfaces was evaluated by tartrate resistant acid phosphatase (TRAP) staining and cathepsin K immunohistochemistry. On day 3, the number of TRAP- and cathepsin K-positive cells increased more so in the PD group than in the C group. The PD+infliximab group showed a lower number of positive cells than the PD group. There was no difference in all the groups on day 20. On day 3, the cathepsin-K positive multinucleated and mononucleated cells were higher in the PD group than in the C group. The number of cathepsin-K positive multinucleated cells was lower in the PD+infliximab group than in the PD group. The PD group showed more cathepsin K-positive cells in the furcation and distal surfaces than the c group. The Cathepsin K-positive cells of the PD+infliximab group were lower than that of the PD group in furcation. These results suggest that TNF-α stimulates odontoclast formation in diabetes with periodontitis.
