Development of Competitive ELISA for Detection of Avian Metapneumovirus Antibodies in Chicken.
10.4167/jbv.2010.40.3.131
- Author:
Kang Seuk CHOI
1
;
Jin Won KIM
;
Eun Kyoung LEE
;
Woo Jin JEON
;
Mi Ja PARK
;
Yeh Na LYOO
;
Jun Hun KWON
Author Information
1. Avian Diseases Division, National Veterinary Research and Quarantine Service, Anyang, Korea. kchoi0608@korea.kr
- Publication Type:Original Article
- Keywords:
Avian metapneumovirus;
ELISA;
N protein;
Antibody detection
- MeSH:
Antibodies;
Baculoviridae;
Binding, Competitive;
Birds;
Chickens;
Egg Yolk;
Enzyme-Linked Immunosorbent Assay;
Mass Screening;
Metapneumovirus;
Neutralization Tests;
Respiratory Tract Infections;
Sensitivity and Specificity;
Sprains and Strains;
Turkeys;
Viruses
- From:Journal of Bacteriology and Virology
2010;40(3):131-143
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Avian metapneumovirus (aMPV) causes an acute and highly contagious upper respiratory tract infection in turkeys and chickens. In this study, a competitive ELISA (C-ELISA) was developed for the detection of antibodies to aMPV in chicken sera and/or their egg yolks. This assay is based on the competitive binding of monoclonal antibody with serum antibodies to recombinant aMPV N protein expressed by a recombinant baculovirus. The C-ELISA showed specificity and sensitivity of 100% and 98.0%, respectively, when compared to the virus neutralization test. In specific pathogen-free chickens experimentally infected with aMPV SC1509 strain, the C-ELISA started to detect antibodies to aMPV as early as 5 days post infection from birds infected with aMPV, while a commercial ELISA kit detected first 10 days post infection. The C-ELISA was similar or superior to a commercial ELISA kit when serum and egg yolk samples collected from chickens on six outbreak farms were tested for diagnosis. The C-ELISA developed in the present work provides a short turnaround time and can be a useful diagnostic and screening tool for aMPV infection in the field.
