The Minimal Inhibitory Concentrations of Antifungal Agents for Clinical Isolates in Fungal Keratitis.
- Author:
Seung Wan SOHN
1
;
Young Ho HAHN
Author Information
1. Department of Ophthalmology, Gospel Hospital, The Kosin University, Pusan, Korea.
- Publication Type:Original Article ; In Vitro
- Keywords:
Antifungal agent;
Fungal keratitis;
Microscopic minimal inhibitory concentration[MIC];
Susceptibility test
- MeSH:
Amphotericin B;
Antifungal Agents*;
Clotrimazole;
Fungi;
Itraconazole;
Keratitis*;
Ketoconazole;
Miconazole;
Powders;
Spores, Fungal
- From:Journal of the Korean Ophthalmological Society
2000;41(5):1054-1061
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed for the standardization and proper selection of effective antifungal agents by measuring the minimal inhibitory concentra-tions[MICs]of antifungals to fungi, separated from keratitis patients.Two strains of A.fumigatus and single strain of F.solani, A.falciforme, and A . alternata were used for this test.Standard powders of miconazole, itraconazole, clotrimazole, ketoconazole, and amphotericin B were used as antifungal agents. Microscopic and macroscopic measurements of MIC after 24, 48 and 72 hours of inoculation[105 conidia /ml]into YNB broth with culture temperature of 25 degrees C were performed by use of broth microdilution method.The results are as follows : itraconazole, amphotericin B, and clotrimazole were effective against A.fumigatus.F.solani showed resistance to all kinds of antifungal agents.A.falciforme and A.alternata were sensitive to amphotericin B and itraconazole, respectively. Further studies may be needed for the standardized measurement of MIC against filamentous fungi and also for in Vitro-in Vivo correlations for the treatment of fungal keratitis.
