Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing NF-kappaB and MAPK Activation.
- Author:
Byoung Ok CHO
1
;
Hyung Won RYU
;
Yangkang SO
;
Chang Wook LEE
;
Chang Hyun JIN
;
Hong Sun YOOK
;
Yong Wook JEONG
;
Jong Chun PARK
;
Il Yun JEONG
Author Information
1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea. iyjeong@kaeri.re.kr
- Publication Type:Original Article
- Keywords:
Mangostenone F;
NO;
iNOS;
NF-kappaB;
MAPK
- MeSH:
Cytokines;
DNA;
Down-Regulation;
Garcinia mangostana;
Interleukin-6;
Luciferases;
Macrophages*;
NF-kappa B*;
Phosphorylation;
Phosphotransferases;
Transcription Factor AP-1
- From:Biomolecules & Therapeutics
2014;22(4):288-294
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mangostenone F (MF) is a natural xanthone isolated from Garcinia mangostana. However, little is known about the biological activities of MF. This study was designed to investigate the anti-inflammatory effect and underlying molecular mechanisms of MF in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. MF dose-dependently inhibited the production of NO, iNOS, and pro-inflammatory cytokines (TNF-alpha, IL-6, and IL-1beta) in LPS-stimulated RAW264.7 macrophages. Moreover, MF decreased the NF-kappaB luciferase activity and NF-kappaB DNA binding capacity in LPS-stimulated RAW264.7 macrophages. Furthermore, MF suppressed the NF-kappaB activation by inhibiting the degradation of IkappaBalpha and nuclear translocation of p65 subunit of NF-kappaB. In addition, MF attenuated the AP-1 luciferase activity and phosphorylation of ERK, JNK, and p38 MAP kinases. Taken together, these results suggest that the anti-inflammatory effect of MF is associated with the suppression of NO production and iNOS expression through the down-regulation of NF-kappaB activation and MAPK signaling pathway in LPS-stimulated RAW264.7 macrophages.
