Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and 12-PGJ2..
- Author:
Sang Gun AHN
1
;
Ho Shik KIM
;
Seong Whan JEONG
;
Bo Eun KIM
;
Hyang Shuk RHIM
;
Jae Yong SHIM
;
Jin Woo KIM
;
Jeong Hwa LEE
;
In Kyung KIM
Author Information
1. Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Sox-4;
PGA2;
delta(12)-PGJ2;
apoptosis
- MeSH:
Apoptosis/*drug effects;
Blotting, Western;
Calcimycin/pharmacology;
Caspase 1/antagonists & inhibitors/metabolism;
Etoposide/pharmacology;
Gene Expression Regulation, Neoplastic/drug effects;
High Mobility Group Proteins/genetics/*metabolism;
Human;
Liver Neoplasms/enzymology/metabolism/pathology;
Oligopeptides/pharmacology;
Prostaglandin D2/*analogs & derivatives/*pharmacology;
Prostaglandins A/*pharmacology;
Trans-Activators/genetics/*metabolism;
Transfection;
Tumor Cells, Cultured
- From:Experimental & Molecular Medicine
2002;34(3):243-249
- CountryRepublic of Korea
- Language:English
-
Abstract:
We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and delta(12)-PGJ(12) induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca(2+) ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA(2) and delta(12)-PGJ(12) in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.
