Chemopreventive and metabolic effects of inulin on colon cancer development.
10.4142/jvs.2013.14.4.387
- Author:
Emilia HIJOVA
1
;
Viktoria SZABADOSOVA
;
Jana STOFILOVA
;
Gabriela HRCKOVA
Author Information
1. Institute of Experimental Medicine, Faculty of Medicine, Pavol Jozef Safarik University, Kosice 040 11, The Slovak Republic. emilia.hijova@upjs.sk
- Publication Type:Original Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
- Keywords:
chemoprevention;
colon cancer;
prebiotic;
Sprague-Dawley rats
- MeSH:
Animals;
Bacterial Proteins/genetics/metabolism;
Colon/enzymology;
Colonic Neoplasms/chemically induced/*drug therapy/metabolism;
Colony Count, Microbial;
Cyclooxygenase 2/genetics/metabolism;
Cytokines/blood/genetics;
Diet;
Dietary Supplements/analysis;
Dimethylhydrazines/toxicity;
Enterobacteriaceae/drug effects/physiology;
Fatty Acids, Volatile/genetics/metabolism;
Female;
Gene Expression Regulation/drug effects;
Inulin/administration & dosage/*metabolism;
Lactobacillaceae/drug effects/physiology;
Male;
NF-kappa B/genetics/metabolism;
Prebiotics/*analysis;
Rats;
Rats, Sprague-Dawley
- From:Journal of Veterinary Science
2013;14(4):387-393
- CountryRepublic of Korea
- Language:English
-
Abstract:
Prebiotics modulate microbial composition and ensure a healthy gastrointestinal tract environment that can prevent colon cancer development. These natural dietary compounds are therefore potential chemopreventive agents. Thirty Sprague-Dawley rats (4 months old) were experimentally treated with procarcinogen dimethylhydrazine to induce colon cancer development. The rats were randomly assigned to three groups: a control group (CG), a group treated with dimethylhydrazine (DMH), and a group given DMH and inulin, a prebiotic (DMH+PRE). The effects of inulin on the activities of bacterial glycolytic enzymes, short-chain fatty acids, coliform and lactobacilli counts, cytokine levels, and cyclooxygenase-2 (COX-2) and transcription nuclear factor kappa beta (NFkappaB) immunoreactivity were measured. Inulin significantly decreased coliform counts (p < 0.01), increased lactobacilli counts (p < 0.001), and decreased the activity of beta-glucuronidase (p < 0.01). Butyric and propionic concentrations were decreased in the DMH group. Inulin increased its concentration that had been reduced by DMH. Inulin decreased the numbers of COX-2- and NFkappaB-positive cells in the tunica mucosae and tela submucosae of the colon. The expression of IL-2, TNFalpha, and IL-10 was also diminished. This 28-week study showed that dietary intake of inulin prevents preneoplastic changes and inflammation that promote colon cancer development.
