Alpha-Type 1 Polarized Dendritic Cells Loaded with Apoptotic Allogeneic Breast Cancer Cells Can Induce Potent Cytotoxic T Lymphocytes against Breast Cancer.
- Author:
Min Ho PARK
1
;
Deok Hwan YANG
;
Mi Hyun KIM
;
Jae Hong JANG
;
Yoon Young JANG
;
Youn Kyung LEE
;
Chun Ji JIN
;
Than Nhan Nguyen PHAM
;
Truc Anh Nguyen THI
;
Mi Seon LIM
;
Hyun Ju LEE
;
Cheol Yi HONG
;
Jung Han YOON
;
Je Jung LEE
Author Information
1. Department of Surgery, Chonnam National University Hwasun Hospital, Chonnam National University School of Medicine, Hwasun, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Dendritic cells;
Breast neoplasms;
Allogeneic;
Cytotoxic T lymphocytes;
Immunotherapy
- MeSH:
Antigens, Neoplasm;
Breast;
Breast Neoplasms;
CD40 Ligand;
Cell Line;
Dendritic Cells;
Humans;
Immunotherapy;
Interleukin-12;
Lymphocytes;
T-Lymphocytes, Cytotoxic
- From:Cancer Research and Treatment
2011;43(1):56-66
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Various tumor antigens can be loaded onto dendritic cells (DCs) to induce a potent cytotoxic T lymphocyte (CTL) response in DC-based immunotherapy against breast cancer. However, in the clinical setting, obtaining a sufficient number of autologous tumor cells as a source of tumor antigens is a laborious process. We therefore investigated the feasibility of immunotherapy using breast-cancer-specific CTLs generated in vitro by use of alpha-type 1 polarized DCs (alpha DC1s) loaded with ultraviolet B-irradiated cells of the breast cancer cell line MCF-7. MATERIALS AND METHODS: alphaDC1s were induced by loading allogeneic tumor antigen generated from the MCF-7 UVB-irradiated breast cancer cell line. Antigen-pulsed alphaDC1s were evaluated by morphological and functional assays, and the breast-cancer-specific CTL response was analyzed by cytotoxic assay. RESULTS: The alphaDC1s significantly increased the expression of several molecules related to DC maturation without differences according to whether the alphaDC1s were loaded with tumor antigens. The alphaDC1s showed a high production of interleukin-12 both during maturation and after subsequent stimulation with CD40L, which was not significantly affected by loading with tumor antigens. Breast-cancer-specific CTLs against autologous breast cancer cells were successfully induced by alphaDC1s loaded with apoptotic MCF-7 cells. CONCLUSION: Autologous DCs loaded with an allogeneic breast cancer cell line can generate potent breast-cancer-specific CTL responses. This may be a practical method for cellular immunotherapy in patients with breast cancer.
