Ovarian cancer related gene targeting with large circular antisense library.
- Author:
Kyung Oh DOH
1
;
Geun Soo CHUN
;
Jae Chul SIM
;
Hoe Saeng YANG
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine Dongguk University, Gyeongju, Korea. y31354@hotmail.com
- Publication Type:Original Article
- Keywords:
Ovarian neoplasms;
Large circular antisense DNA;
Gene therapy
- MeSH:
Bacteriophages;
Clone Cells;
Estrone;
Gene Targeting;
Genetic Therapy;
Ovarian Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
2009;52(7):724-733
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study is to find out the genes which are related to ovarian cancer cell growth using large circular antisense library. METHODS: Clones for antisense library were uni-directionally sub-cloned into pBS SK (-) vector. LC-antisense molecules were then purified from the culture supernatants of the bacterial competent cells superinfected with M13K07 helper bacteriophages. The LC-antisense library to 240 unigene clone was constructed and utilized in the identification of genes functionally involved in the growth of ovarian cancer cells. RESULTS: The 17 numbers out of the 240 numbers of the antisense library exerted a marked inhibitory effect on the growth of SK-OV 3. CONCLUSION: The putative functional categorization of each gene was then conducted via public databases. These candidates may be used as target genes for drug development or adjuvant of conventional chemotherapeutic drugs.
