Effects of 630 nm Light-Emitting Diode Irradiation on Caveolin-1 and Procollagen I and III Expression in Human Dermal Fibroblasts.
- Author:
Da In CHOI
1
;
Jee Young CHOI
;
Sang Yub KIM
;
Suk Jung YUN
;
Jee Bum LEE
;
Seung Chul LEE
Author Information
1. Department of Dermatology, Chonnam National University Medical School, Gwangju, Korea. schul@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
Antiaging activity;
Caveolin-1;
Light emitting diode (LED);
Procollagens I and III
- MeSH:
Caveolin 1*;
Collagen;
Fibroblasts*;
Humans;
Metabolism;
Procollagen*;
RNA, Messenger;
RNA, Small Interfering;
Skin;
Transforming Growth Factors;
Up-Regulation
- From:Korean Journal of Dermatology
2014;52(3):161-168
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recent studies indicate that light-emitting diodes (LED) may represent a novel and effective anti-aging light source for the skin. Among many candidate molecules known to control collagens, caveolin-1 (Cav-1) is known to play an inhibitory role in cutaneous collagen metabolism. OBJECTIVE: This study aimed to evaluate the effects of LED irradiation on the expression levels of Cav-1 and procollagens (proCOLs) in human dermal fibroblasts (HDFs). METHODS: Cultured HDFs were irradiated with 630 nm LED at different doses, and the mRNA and protein expression levels of Cav-1 and proCOLs I/III were analyzed. RESULTS: In LED-irradiated HDFs, mRNA and protein levels of Cav-1 were found to be down-regulated, whereas those of proCOLs I/III were up-regulated in a dose-dependent manner. A negative correlation between Cav-1 and proCOLs was verified in Cav-1 siRNA transfected HDFs. LED was moreover found to result in up-regulation of transforming growth factor (TGF)-beta1 and its receptors (TbetaRI, TbetaRII), SMAD1, and SMAD2 mRNA levels, indicating that LED may activate the TGF-1/TbetaR/SMAD pathway in HDFs. CONCLUSION: The anti-aging effects of 630 nm LED on human skin are likely mediated by up-regulation of proCOLs I/III and inhibition of Cav-1 expression levels in HDFs.
