nm23-H1 Protein Expression and Gene Mutation in 150 Patients with Non-Hodgkin's Lymphomas.
10.3346/jkms.2006.21.4.645
- Author:
Ju Han LEE
1
;
Su Jin CHO
;
Xianglan ZHANG
;
Zhenlong ZHENG
;
Eung Seok LEE
;
Aeree KIM
;
Young Sik KIM
;
Yang Seok CHAE
;
Insun KIM
Author Information
1. Department of Pathology, College of Medicine, Korea University, Seoul, Korea. iskim@korea.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
nucleoside diphosphate kinase A;
nm23-H1 Protein;
Lymphoma, Non-Hodgkin;
Mutation;
Prog-nosis
- MeSH:
Tissue Array Analysis;
Survival Analysis;
Prognosis;
Polymorphism, Single-Stranded Conformational;
Nucleoside-Diphosphate Kinase/*genetics/metabolism;
Mutation/*genetics;
Middle Aged;
Male;
Lymphoma, T-Cell/genetics/metabolism/pathology;
Lymphoma, Non-Hodgkin/genetics/metabolism/*pathology;
Lymphoma, Mantle-Cell/genetics/metabolism/pathology;
Lymphoma, Large-Cell, Diffuse/genetics/metabolism/pathology;
Lymphoma, B-Cell/genetics/metabolism/pathology;
Immunohistochemistry;
Humans;
Female;
DNA Mutational Analysis;
Base Sequence
- From:Journal of Korean Medical Science
2006;21(4):645-651
- CountryRepublic of Korea
- Language:English
-
Abstract:
The metastasis-suppressing role of the nm23 gene in the metastatic spread of malignant tumor is still debated. We examined the nm23-H1 protein expression and gene mutation in non-Hodgkin's lymphomas to compare with the clinicopathologic parameters. The expression of nm23-H1 protein was immunohistochemically examined in 150 cases of non-Hodgkin's lymphomas; 85 diffuse large B cell lymphomas (DL-BCL), 18 marginal zone B cell lymphomas (MZL), 3 mantle cell lymphomas, 25 peripheral T cell lymphomas, not otherwise specified (TCLNOS), and 19 NK/T cell lymphomas (NK/T). Eighty-one cases (58 DLBCL, 6 MZL, 4 TCLNOS, and 13 NK/T) were studied for nm23-H1 gene mutation in exon 1 to 5. The high expression of nm23-H1 protein was associated with the high IPI score (p=0.019) and the low survival rate of the patients (p=0.0039). The gene mutation of nm23-H1 was detected in 10.3% of DLBCL and 30.7% of NK/T; but none in MZL and TCLNOS. The mutation was found in exon 1 in 5 cases, exon 2 in two cases, exon 4 in one case and both exon 1 and 2 in two cases. Our results suggest that the expression of nm23-H1 protein can be used as a poor prognostic marker in non-Hodgkin's lymphomas, and the mutational change of gene may operate in the lymphomagenesis.
