Molecular Epidemiologic Analysis of Methicillin-Resistant Staphylococcus aureus Isolates from Bacteremia and Nasal Colonization at 10 Intensive Care Units: Multicenter Prospective Study in Korea.
10.3346/jkms.2011.26.5.604
- Author:
Jae Cheol KWON
1
;
Si Hyun KIM
;
Sun Hee PARK
;
Su Mi CHOI
;
Dong Gun LEE
;
Jung Hyun CHOI
;
Chulmin PARK
;
Na Young SHIN
;
Jin Hong YOO
Author Information
1. Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. jhyoo@catholic.ac.kr
- Publication Type:Original Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
- Keywords:
Methicillin-Resistant Staphylococcus aureus;
Intensive Care Units;
Bacteremia;
Molecular Epidemiology
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Bacteremia/*epidemiology/microbiology;
Cross Infection/epidemiology/genetics;
Drug Resistance, Multiple, Bacterial;
Electrophoresis, Gel, Pulsed-Field;
Female;
Genotype;
Humans;
*Intensive Care Units;
Male;
Methicillin-Resistant Staphylococcus aureus/*genetics/isolation & purification;
Microbial Sensitivity Tests;
Middle Aged;
Molecular Epidemiology;
Molecular Typing;
Nasal Lavage Fluid/*microbiology;
Prospective Studies;
Republic of Korea/epidemiology;
Staphylococcal Infections/*epidemiology/genetics/microbiology
- From:Journal of Korean Medical Science
2011;26(5):604-611
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) isolated at 10 intensive care units (ICUs) in Korea. MRSA isolates from bacteremia and nasal colonization were collected prospectively from October 2008 through May 2009 at 10 University-affiliated hospital ICUs. A total of 83 and 175 MRSA strains were isolated from bacteremia and nasal colonization, respectively. Acquired group accounted for 69.9% (n = 58) of bacteremia and 73.1% (n = 128) of nasal colonization. Pulsed-field gel electrophoresis (PFGE) type B (SCCmec type II/ST5) was dominant in the acquired group followed by PFGE type D (SCCmec type IVA/ST72; a community genotype). Seven of 58 (12.1%) acquired bacteremia and 15 of 128 (11.8%) acquired nasal colonizations had SCCmec type IVA/ST72 genotype, which indicated that the community genotype had already emerged as a cause of ICU acquired MRSA infection or colonization. Antibiotic resistance rates to ciprofloxacin, tetracycline, clindamycin and trimethoprim/sulfamethoxazole were 84.4%, 67.1%, 78.1%, and 12.0%, respectively. Susceptibility to ciprofloxacin best predicted a community genotype (sensitivity 96.5%; specificity 96.9%; odds ratio 861; 95% confidence interval 169-4,390, P < 0.001) and the positive predictive value was 90.2%. Among 23 nasal re-colonized strains, 7 MRSA strains (30.4%) were different from the originally colonized strains on the basis of PFGE types.
