Exposure to Toluene Diisocyanate (TDI) Induces IL-8 Production from Bronchial Epithelial Cells: Effect of Pro-inflammatory Cytokines.
10.3346/jkms.2003.18.6.809
- Author:
Young Mok LEE
1
;
Hyun Ah KIM
;
Hae Sim PARK
;
Soo Keol LEE
;
Dong Ho NAHM
Author Information
1. Department of Allergy & Rheumatology, Ajou University School of Medicine, Suwon, Korea. hspark@madang.ajou.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Isocyanates;
TDI-Asthma;
Epithelial Cells;
Interleukin-8;
Interleukin-1;
Dexamethasone
- MeSH:
Asthma/immunology;
Bronchi/*cytology/metabolism;
Cell Line;
Dexamethasone/pharmacology;
Epithelial Cells/cytology/*drug effects/*metabolism;
Glucocorticoids/pharmacology;
Human;
Interleukin-8/*metabolism;
Leukocytes, Mononuclear/cytology/metabolism;
Support, Non-U.S. Gov't;
Toluene 2,4-Diisocyanate/*toxicity
- From:Journal of Korean Medical Science
2003;18(6):809-812
- CountryRepublic of Korea
- Language:English
-
Abstract:
This investigation was designed to confirm IL-8 production from human bronchial epithelial cells with toluene diisocyanate (TDI) exposure and to examine the effects of pro-inflammatory cytokine and dexamethasone. We cultured Beas-2B, a bronchial epithelial cell line with TDI-HSA conjugate and compared with those without conjugate. IL-8 in the supernatant was measured by ELISA. To evaluate the effect of proinflammatory cytokines, peripheral blood mononuclear cells (PBMC) were collected from TDI- and non-TDI asthma patients, and were added to the epithelial cell culture. Dexamethasone or antibodies to TNF-alpha and IL-1beta were pre-incubated with PBMC supernatant. There was a significant production of IL-8 from bronchial epithelial cells with addition of TDI-HSA conjugate in a dose-dependent manner, which was significantly augmented with addition of PBMC supernatant. Higher production of IL-8 was noted with addition of PBMC supernatant from TDI-asthma patients than in those from non-TDI asthma patients. IL-1beta and IL-1beta/TFNalpha antibodies were able to suppress the IL-8 productions. Pre-treatment of dexamethasone induced dose-dependent inhibition of the IL-8 production. These results suggest that the IL-8 production from bronchial epithelial cells contribute to neutrophil recruitment occurring in TDIinduced airway inflammation. IL-1beta released from PBMC of TDI-induced asthma patients may be one of the pro-inflammatory cytokines to enhance IL-8 production.
