Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate.
10.4142/jvs.2011.12.4.401
- Author:
Min Kyoung SHIN
1
;
Myung Hwan JUNG
;
Won Jung LEE
;
Pil Son CHOI
;
Yong Suk JANG
;
Han Sang YOO
Author Information
1. Department of Infectious Disease, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. yoohs@snu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Actinobacillus pleuropneumoniae;
ApxIIA;
cholera toxin B subunit protein;
transgenic plant
- MeSH:
Actinobacillus Infections/microbiology/*prevention & control;
Actinobacillus pleuropneumoniae;
Animals;
Antigens, Bacterial/immunology;
Bacterial Proteins/*immunology;
Bacterial Vaccines/*immunology;
Cholera Toxin/*chemistry;
Female;
Hemolysin Proteins/*immunology;
Immunization, Secondary;
Mice;
Mice, Inbred ICR;
Plants, Genetically Modified;
Zea mays/*genetics
- From:Journal of Veterinary Science
2011;12(4):401-403
- CountryRepublic of Korea
- Language:English
-
Abstract:
Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.
