The Effects of Glutathione on Human Cancer Cells before and after the Application of Cisplatin.
- Author:
Young Shin CHO
1
;
Hyoung Gyun ROH
;
Tae Gyu AHN
;
Sei Jun HAN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Kwangju, Korea.
- Publication Type:Original Article
- Keywords:
Cisplatin;
Cervical carcinoma cell;
Glutathione;
OTC
- MeSH:
Cisplatin*;
Glutathione*;
Humans*
- From:Korean Journal of Obstetrics and Gynecology
2004;47(1):161-165
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of this study is to determine the time-dependant effects of Glutathione on the Cisplatin-induced cytotoxicity of human cervical carcinoma cells. METHODS: Two human cervical carcinoma cells, SiHa (squamous cell carcinoma cell), and CaSki (epidermoid metastatic carcinoma cell) were cultivated with RPMI1640 media. Reduced glutathione (GSH) and 2-oxo 4-thiazolidine carboxylic acid (OTC) were added one hour before and after Cisplatin (2-50 micro M/ml) was applied. The cells were incubated an additional 24 hours and viable cells were examined using a 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: The MTT reduction rate of cisplatin-treated cervical carcinoma cells was increased significantly by the addition of glutathione (5 mM) or OTC (5 mM) both one hour before and after Cisplatin. A difference between MTT reduction rates one hour before and after cisplatin were not observed in either GSH or OTC. CONCLUSION: These results suggest that GSH and OTC have a protective effect on cisplatin-induced toxicity, and that this effect is about the same whether the agents were applied before or after the Cisplatin.
