PMK-S005 Alleviates Age-Related Gastric Acid Secretion, Inflammation, and Oxidative Status in the Rat Stomach.
- Author:
Yoon Jeong CHOI
1
;
Nayoung KIM
;
Ju Yup LEE
;
Ryoung Hee NAM
;
Ji Hyung SUH
;
Sun Min LEE
;
Min Hee HAM
;
Hyun Jin JO
;
Young Kwang SHIM
;
Yo Han PARK
;
Jong Chan LEE
;
Yoon Jin CHOI
;
Hye Seung LEE
;
Dong Ho LEE
Author Information
- Publication Type:Original Article
- Keywords: s-allyl-l-cysteine; Aging; Gastric acid; Anti-inflammation; Antioxidants
- MeSH: Aging; Animals; Antioxidants; Blotting, Western; Child, Preschool; Connective Tissue; Diet; Enzyme-Linked Immunosorbent Assay; Gastric Acid*; Heme; Humans; Inflammation*; Interleukins; Lansoprazole; Mucous Membrane; Oxidative Stress; Rats*; Stomach*; Tumor Necrosis Factor-alpha
- From:Gut and Liver 2016;10(5):749-756
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS: The aim of this study was to evaluate the effect of the synthetic S-allyl-l-cysteine (SAC) PMK-S005 on gastric acid secretion, inflammation, and antioxidant enzymes in aging rats. METHODS: The rats were divided into four groups at 31 weeks of age and were continuously fed a diet containing a vehicle control, PMK-S005 (5 or 10 mg/kg), or lansoprazole (5 mg/kg). Gastric acid secretion and connective tissue thickness of the lamina propria were evaluated at 74 weeks and 2 years of age. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and COX-2 levels were measured by using enzyme-linked immunosorbent assays (ELISAs) or Western blot assays. Levels of antioxidant enzymes, including heme oxyganase 1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO-1), were also measured. RESULTS: As the rats aged, gastric acid secretion significantly decreased, and the connective tissue of the lamina propria increased. However, 74-week-old rats in the PMK-S005 group exhibited greater levels of gastric acid secretion than those of the control and lansoprazole groups. The increase of TNF-α, IL-1β, and COX-2 expression in 74-week and 2-year-old control rats were inhibited by PMK-S005. In addition, the decrease in HO-1 and NQO-1 protein expression that occurred with aging was inhibited by PMK-S005 in the 74-week-old rats. CONCLUSIONS: These results suggest that PMK-S005 has therapeutic potential as an antiaging agent to ameliorate age-related gastric acid secretion, inflammation, and oxidative stress in the stomach.
