Tissue Microarray Analysis of Fas and FasL Expressions in Human Non-small Cell Lung Carcinomas; with Reference to the p53 and bcl-2 Overexpressions.
10.3346/jkms.2005.20.5.770
- Author:
Na Hye MYONG
1
Author Information
1. Department of Pathology, Dankook University College of Medicine, Cheonan, Korea. myongnh@hanmail.net
- Publication Type:Original Article ; Comparative Study ; Controlled Clinical Trial ; Research Support, Non-U.S. Gov't
- Keywords:
Antigens, CD95;
FasL protein;
Carcinoma, Non-Small-Cell Lung;
Carcinogenesis;
Tissue Array Analysis
- MeSH:
Antigens, CD95/*metabolism;
Apoptosis;
Carcinoma, Non-Small-Cell Lung/*metabolism/mortality;
Cell Survival;
Comparative Study;
Female;
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic;
Humans;
Korea/epidemiology;
Lung Neoplasms/*metabolism/mortality/pathology;
Male;
Membrane Glycoproteins/*metabolism;
Middle Aged;
Oligonucleotide Array Sequence Analysis;
Prognosis;
Proto-Oncogene Proteins c-bcl-2/*metabolism;
Research Support, Non-U.S. Gov't;
Risk Assessment/methods;
Risk Factors;
Survival Analysis;
Survival Rate;
Tumor Markers, Biological/*metabolism;
Tumor Necrosis Factors/*metabolism;
Tumor Suppressor Protein p53/*metabolism
- From:Journal of Korean Medical Science
2005;20(5):770-776
- CountryRepublic of Korea
- Language:English
-
Abstract:
Lack of surface Fas expression is a main route for apoptotic resistance which is considered an important mechanism of tumorigenesis and tumor progression. Fas and FasL expressions in 110 non-small cell lung carcinomas (NSCLCs) were investigated to evaluate their roles in pulmonary carcinogenesis and to examine the clinicopathologic significance of Fas expression with its relationship with p53 and bcl-2 overexpressions. Immunohistochemical analysis using tissue microarray demonstrated that a large proportion of NSCLC patients (60%) showed lack of membranous Fas expression. The Fas-negative cases revealed the significantly lower survival rate than Fas-positive ones. Also, the loss of Fas receptor expression was found more frequently in advanced stage and higher nodal status. FasL protein was increased in most NSCLCs (89%) compared to normal lungs. p53 and bcl-2 overexpressions showed no association with Fas expression. Conclusively, reduced membranous Fas expression as a mechanism of apoptotic resistance is considered to play an important part of the pulmonary carcinogenesis, which may predict poor survival and have a bad prognostic influence. Increased FasL expression is thought to be a basis for the immune evasion in NSCLCs. The rare bcl-2 overexpression suggests that this anti-apoptotic protein is unlikely to play a role in the apoptotic resistance of NSCLCs.
