Labeling Efficacy of Superparamagnetic Iron Oxide Nanoparticles to Human Neural Stem Cells: Comparison of Ferumoxides, Monocrystalline Iron Oxide, Cross-linked Iron Oxide (CLIO)-NH2 and tat-CLIO.
- Author:
Miyeoun SONG
1
;
Woo Kyung MOON
;
Yunhee KIM
;
Dongyeol LIM
;
In Chan SONG
;
Byung Woo YOON
Author Information
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords: Human neural stem cell; Iron oxide nanoparticles; Magnetic resonance (MR)
- MeSH: Cells, Cultured; Contrast Media/chemical synthesis/pharmacokinetics; Cross-Linking Reagents/chemistry; Ferric Compounds/chemistry/*pharmacokinetics; Ferrosoferric Oxide/chemical synthesis/pharmacokinetics; Gene Products, tat/chemistry; Humans; Iron/*pharmacokinetics; Magnetic Resonance Imaging/methods; Nanoparticles; Neural Tube; Oxides/*pharmacokinetics; Phantoms, Imaging; Polylysine/pharmacokinetics; Spectrophotometry, Atomic; Staining and Labeling/*methods; Stem Cells/cytology/*drug effects/metabolism; Time Factors; Transfection
- From:Korean Journal of Radiology 2007;8(5):365-371
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVE: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH2 and tat-CLIO. MATERIALS AND METHODS: The hNSCs (5x105 HB1F3 cells/ml) were incubated for 24 hr in cell culture media that contained 25 microgram/ml of ferumoxides, MION or CLIO-NH2, and with or without poly-L-lysine (PLL) and tat-CLIO. The cellular iron uptake was analyzed qualitatively with using a light microscope and this was quantified via atomic absorption spectrophotometry. The visibility of the labeled cells was assessed with MR imaging. RESULTS: The incorporation of SPIONs into the hNSCs did not affect the cellular proliferations and viabilities. The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH2, respectively. However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH2 into the hNSCs was comparable to that of tat-CLIO. CONCLUSION: For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH2 and the transfection agent PLL.
