Isolation of Subgroup J Avian Leukosis Virus in Korea.
- Author:
Haan Woo SUNG
1
;
Jae Hong KIM
;
Sanjay REDDY
;
Aly FADLY
Author Information
1. National Veterinary Research and Quarantine Service, Ministry of Agriculture & Forestry, 480 Anyang 6-Dong, Anyang 430-016, Korea. sunghw@nvrqs.go.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
subgroup J avian leukosis virus;
myeloid leukosis;
virus isolation
- MeSH:
Animals;
Antibodies, Viral/immunology;
Antigens, Viral/immunology;
Avian Leukosis/virology;
Avian leukosis virus/*classification/genetics/immunology/*isolation & purification;
Cell Line;
Chick Embryo;
Chickens/*virology;
Korea;
Neutralization Tests;
Polymerase Chain Reaction;
Poultry Diseases/virology
- From:Journal of Veterinary Science
2002;3(2):71-74
- CountryRepublic of Korea
- Language:English
-
Abstract:
Two subgroup J avian leukosis viurses (ALVs) were isolated from broiler breeder flocks, in which myeloid leukosis had occurred. The isolates could be classified as subgroup J ALV. by the positive reaction in polymerase chain reaction (PCR) with primers specific for subgroup J ALV. Two isolates replicated in chicken embryo fibroblast (CEF) cells from the alv6 chicken line in which cells are resistant to subgroup A and E ALVs. In in vitro serum neutralization tests with other subgroup ALVs including ADOL-Hc1, the prototype of subgroup J ALVs isolated in the United States of America, two isolates were partially neutralized by antibody to ADOL-Hc1, indicating that Korean isolates and ADOL-Hc1 may be antigenically related, but not identical. When the PCR was done with a primer pair designed to amplify genes of E element and long terminal repeat of proviral DNA, the PCR product size of one isolate (KOAL-PET) was smaller than that of ADOL-Hc1, suggesting that some sequences in these regions are deleted.
