Role of tissue inhibitors of metalloproteinases (TIMPs) in colorectal carcinoma.
10.3346/jkms.1999.14.4.417
- Author:
Young Eun JOO
1
;
Kang Seok SEO
;
Jin KIM
;
Hyun Soo KIM
;
Jong Sun REW
;
Chang Soo PARK
;
Sei Jong KIM
Author Information
1. Department of Internal Medicine, Chonnam National University Medical School, Kwangju, Korea.
- Publication Type:Original Article
- Keywords:
Tissue inhibitors of metalloproteinases;
Colorectal neoplasms;
Immunohistochemistry;
In situ hybridization
- MeSH:
Adenocarcinoma/pathology;
Adenocarcinoma/mortality;
Adenocarcinoma/enzymology*;
Adult;
Aged;
Aged, 80 and over;
Antibodies;
Collagenases/immunology;
Collagenases/genetics*;
Collagenases/analysis;
Colorectal Neoplasms/pathology;
Colorectal Neoplasms/mortality;
Colorectal Neoplasms/enzymology*;
DNA Probes;
Female;
Gelatinase A;
Gelatinase B;
Gelatinases/immunology;
Gelatinases/genetics*;
Gelatinases/analysis;
Gene Expression Regulation, Enzymologic;
Gene Expression Regulation, Neoplastic;
Human;
In Situ Hybridization;
Male;
Metalloendopeptidases/immunology;
Metalloendopeptidases/genetics*;
Metalloendopeptidases/analysis;
Middle Age;
Predictive Value of Tests;
RNA, Messenger/analysis;
Stromal Cells/pathology;
Stromal Cells/enzymology;
Survival Analysis;
Tissue Inhibitor-of Metalloproteinase-2/immunology;
Tissue Inhibitor-of Metalloproteinase-2/genetics*;
Tissue Inhibitor-of Metalloproteinase-2/analysis;
Tissue-Inhibitor of Metalloproteinase-1/immunology;
Tissue-Inhibitor of Metalloproteinase-1/genetics*;
Tissue-Inhibitor of Metalloproteinase-1/analysis
- From:Journal of Korean Medical Science
1999;14(4):417-423
- CountryRepublic of Korea
- Language:English
-
Abstract:
Increased production of matrix metalloproteinases (MMPs) has been associated with increases in invasive and metastatic potential in many types of human carcinoma. Tissue inhibitors of metalloproteinase (TIMP)-1 inhibits most interstitial collagenases and MMP-9. TIMP-2 binds specifically and noncovalently to the pro-form of MMP-2 and inhibits its enzyme activity. In this study, we examined TIMP-1 and TIMP-2 expressions in relation to clinicopathological variables in colorectal carcinoma with in situ hybridization and immunohistochemistry. TIMP-1 and TIMP-2 expressions were localized overwhelmingly to pericancer stromal cells, while malignant and normal mucosal cells were weak or negative. Strong stromal TIMP-1 immunoreactivity correlated with Dukes' stage (p=0.022), status of lymph node metastasis (p=0.044) and poor survival (p= 0.005). The degree of immunohistochemical staining of TIMP-2 did not correlate with all clinicopathological variables. The correlation between enhanced TIMP-1 expression and advanced stage and poor survival suggest a growth promoting activity of TIMP-1 in colorectal carcinoma.
