Expression levels of heat shock protein 27 and cellular FLICE-like inhibitory protein in prostate cancer correlate with Gleason score sum and pathologic stage.
10.4111/kju.2015.56.7.505
- Author:
Seung Wook LEE
1
;
Jeoung Man CHO
;
Hee Ju CHO
;
Jung Yoon KANG
;
Eun Kyung KIM
;
Tag Keun YOO
Author Information
1. Department of Urology, Hanyang University Guri Hospital, Hanyang University School of Medicine, Guri, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Clinical pathology;
Heat-shock proteins;
Neoplasm grading;
Prostatic neoplasms
- MeSH:
Aged;
Biomarkers, Tumor/*metabolism;
CASP8 and FADD-Like Apoptosis Regulating Protein/*metabolism;
HSP27 Heat-Shock Proteins/*metabolism;
Humans;
Lymphatic Metastasis;
Male;
Middle Aged;
Neoplasm Grading;
Neoplasm Proteins/metabolism;
Neoplasm Staging;
Prostatectomy/methods;
Prostatic Hyperplasia/metabolism/surgery;
Prostatic Neoplasms/*metabolism/pathology/surgery;
Transurethral Resection of Prostate
- From:Korean Journal of Urology
2015;56(7):505-514
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Heat shock protein (HSP) 27 protects the cell by controlling apoptosis and immune reactions, and c-FLIP (cellular-FLICE inhibitory protein) inhibits apoptosis by inhibiting caspase-8 activity. We investigated the relationship of HSP27 and c-FLIP expression to prostate-specific antigen, Gleason score sum (GSS), and pathologic stage. MATERIALS AND METHODS: Samples from 163 patients between May 2004 and April 2010 were analyzed: 83 from patients that had underwent a radical prostatectomy, and 80 from those that underwent transurethral resection of the prostate to alleviate urinary symptoms from benign prostate hyperplasia. c-FLIP and HSP27 expression were observed by immunohistochemistry staining. Samples with less than 5% expression-positive cells were scored as 1, with 5%-50% were scored as 2, and with more than 50% were scored as 3. Local reactions were identified as 0.5 and evaluated. RESULTS: Both the presence of HSP27 within the tumor and the number of cancer cells positive for HSP27 were significantly correlated to GSS and pathologic stage (p<0.001, p=0.001, p<0.001, p<0.001). The same was true for c-FLIP expression (p<0.001). GSS was more highly correlated to HSP27 expression than to c-FLIP expression (r=0.814 for HSP27, r=0.776 for c-FLIP), as was pathologic stage (r=0.592 for HSP27, r=0.554 for c-FLIP). CONCLUSIONS: In prostate cancer, higher GSS and a more advanced pathologic stage were associated with a higher likelihood of having a HSP27-positive tumor and more HSP27-positive tumor cells. HSP27 expression was correlated with GSS and prostate cancer stage. A more advanced pathologic stage corresponded to a higher likelihood of having a c-FLIP-positive tumor and more c-FLIP-positive tumor cells. HSP27 expression had a higher correlation with prostate cancer stage and GSS than c-FLIP expression did.
