Effect of Anti-Siglec-F Antibody and Reactive Oxygen Species Blocking on Histamine Release in Urinary Bladder of Ovalbumin-Treated Mice.
- Author:
Bo Hwa CHOI
1
;
Gwoan Youb CHOO
;
Ju Hee KANG
;
Choong Yeol LEE
;
Chang Shin PARK
Author Information
1. Department of Pharmacology, Inha University School of Medicine, BK 21 Project, Incheon, Korea. parkshin@inha.ac.kr
- Publication Type:Original Article
- Keywords:
Histamine;
Mice;
Ovalbumin;
Reactive oxygen species;
Sialic acid binding Ig-like lectin;
Urinary bladder
- MeSH:
Animals;
Histamine;
Histamine Release;
Immunoglobulin G;
Mice;
Ovalbumin;
Ovum;
Plasma;
Reactive Oxygen Species;
Sialic Acid Binding Immunoglobulin-like Lectins;
Urinary Bladder
- From:International Neurourology Journal
2012;16(3):122-125
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Sialic acid-binding Ig-like lectin (Siglec) is an immune inhibitory receptor that plays a role in the negative regulation of the activation of immune cells. This study aimed to evaluate the effects of anti-Siglec-F on plasma and urinary histamine levels in ovalbumin (OVA)-challenged urinary bladder in mice. METHODS: Thirty BALB/c mice were used. In group I (control group, n=5), mice were sensitized with OVA and challenged with saline. In group II (OVA challenge group, n=5), OVA was used for intraperitoneal sensitization and intravesical challenge. The challenged mice in group III (control immunoglobulin G [IgG] group, n=5) and those in group IV (anti-Siglec-F group, n=5) were intraperitoneally pretreated with rabbit control IgG or anti-Siglec-F antibody, respectively. In groups V (N-acetylcysteine [NAC] in OVA challenge group, n=5) and VI (control NAC only, n=5), mice were pretreated with NAC. RESULTS: Urinary histamine concentrations were significantly higher 7 days after intravesical OVA challenge (P<0.01), whereas plasma histamine levels were not. Pretreatment with anti-Siglec-F antibody significantly prevented the increase in urinary histamine release (P<0.05), whereas pretreatment with the IgG antibody control did not. Also, pretreatment of the OVA challenge group with NAC did not affect the histamine concentration in either urine or plasma. CONCLUSIONS: Systemic anti-Siglec-F treatment showed anti-allergic effects at least on local histamine release, particularly in the lower urinary bladder.
