Efficacy of the Mineral Oil and Hyaluronic Acid Mixture Eye Drops in Murine Dry Eye.
10.3341/kjo.2015.29.2.131
- Author:
Jung Han CHOI
1
;
Jung Han KIM
;
Zhengri LI
;
Han Jin OH
;
Kyu Youn AHN
;
Kyung Chul YOON
Author Information
1. Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, Korea. kcyoon@jnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Dry eye;
Hyaluronic acid;
Mineral oil;
Ocular surface
- MeSH:
Animals;
Conjunctiva/*drug effects/pathology;
Cornea/metabolism;
Disease Models, Animal;
Drug Combinations;
Dry Eye Syndromes/*drug therapy/metabolism;
Emollients/administration & dosage;
Female;
Goblet Cells/drug effects/metabolism/pathology;
Hyaluronic Acid/*administration & dosage;
Mice;
Mice, Inbred C57BL;
Mineral Oil/*administration & dosage;
Ophthalmic Solutions;
Tears/*metabolism;
Viscosupplements/administration & dosage
- From:Korean Journal of Ophthalmology
2015;29(2):131-137
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate the therapeutic effects of mineral oil (MO) and hyaluronic acid (HA) mixture eye drops on the tear film and ocular surface in a mouse model of experimental dry eye (EDE). METHODS: Eye drops consisting of 0.1% HA alone or mixed with 0.1%, 0.5%, or 5.0% MO were applied to desiccating stress-induced murine dry eyes. Tear volume, corneal irregularity score, tear film break-up time (TBUT), and corneal fluorescein staining scores were measured at 5 and 10 days after treatment. Ten days after treatment, goblet cells in the conjunctiva were counted after Periodic acid-Schiff staining. RESULTS: There was no significant difference in the tear volume between desiccating stress-induced groups. The corneal irregularity score was lower in the 0.5% MO group compared with the EDE and HA groups. The 0.5% and 5.0% MO groups showed a significant improvement in TBUT compared with the EDE group. Mice treated with 0.1% and 0.5% MO mixture eye drops showed a significant improvement in fluorescein staining scores compared with the EDE group and the HA group. The conjunctival goblet cell count was higher in the 0.5% MO group compared with the EDE group and HA group. CONCLUSIONS: The MO and HA mixture eye drops had a beneficial effect on the tear films and ocular surface of murine dry eye. The application of 0.5% MO and 0.1% HA mixture eye drops could improve corneal irregularity, the corneal fluorescein staining score, and conjunctival goblet cell count compared with 0.1% HA eye drops in the treatment of EDE.
