Real-time Assay of Toxic Lead in In Vivo Living Plant Tissue.
- Author:
Suwyoung LY
1
;
Nack Joo KIM
;
Minsang YOUN
;
Yongwook KIM
;
Yeolmin SUNG
;
Dohoon KIM
;
Tackhyun CHUNG
Author Information
1. Biosensor Research Institute in Seoul National University of Science and Technology, Seoul, Korea. suwyoung@snut.ac.kr
- Publication Type:Original Article
- Keywords:
DNA-CNTPE;
Lead anodic stripping voltammetry;
Low concentration;
In vivo;
Plant;
Tap water
- MeSH:
Electrodes;
Limit of Detection;
Nanotubes;
Plants*;
Water
- From:Toxicological Research
2013;29(4):293-298
- CountryRepublic of Korea
- Language:English
-
Abstract:
A method of detecting lead was developed using square wave anodic stripping voltammetry (SWASV) with DNA-carbon nanotube paste electrode (CNTPE). The results indicated a sensitive oxidation peak current of lead on the DNA-CNTPE. The curves were obtained within a concentration range of 50 ngL-1-20 mgL-1 with preconcentration time of 100, 200, and 400 sec at the concentration of mgL-1, microgL-1, and ngL-1, respectively. The observed relative standard deviation was 0.101% (n = 12) in the lead concentration of 30.0 microgL-1 under optimum conditions. The low detection limit (S/N) was pegged at 8 ngL-1 (2.6 x10-8 M). Results showed that the developed method can be used in real-time assay in vivo without requiring any pretreatment and pharmaceutical samples, and food samples, as well as other materials requiring water source contamination analyses.
