Detection of 12 Respiratory Viruses with Two-set Multiplex Reverse Transcriptase-PCR Assay Using a Dual Priming Oligonucleotide System.
10.3343/kjlm.2007.27.6.420
- Author:
Soo Jin YOO
1
;
Eun Young KUAK
;
Bo Moon SHIN
Author Information
1. Department of Laboratory Medicine, Sanggye Paik Hospital, School of Medicine, Inje University, Seoul, Korea. bmshin@unitel.co.kr
- Publication Type:Original Article
- Keywords:
Respiratory tract infections;
Reverse transcriptase polymerase chain reaction;
Dual priming ologonucleotide system
- MeSH:
Child, Preschool;
DNA, Viral/analysis;
Fluorescent Antibody Technique, Direct;
Humans;
Infant;
Infant, Newborn;
Oligonucleotide Probes;
Reproducibility of Results;
Respiratory Tract Infections/*diagnosis/epidemiology/virology;
Reverse Transcriptase Polymerase Chain Reaction/*methods;
Virus Diseases/*diagnosis/epidemiology/virology;
Viruses/genetics/*isolation & purification
- From:The Korean Journal of Laboratory Medicine
2007;27(6):420-427
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: We intended to evaluate the diagnostic usefulness of a multiplex reverse transcriptase- PCR (mRT-PCR) assay kit under dual priming oligonucleotide system (DPO) for the childhood acute respiratory tract infections. METHODS: Two hundred nasopharyngeal aspirates were taken from children < or = 5 yr old admitted due to acute respiratory infections in 2004. Direct fluorescent antibody (FA) assays were performed with fresh specimens; then, mRT-PCRs for the detection of 12 respiratory viruses (Seeplex RV detection kit, SeeGene, Seoul, Korea) were tested with frozen specimens. RESULTS: FA assays for five common respiratory viruses showed positive results in 66 patients (33.0%), while mRT-PCR detected causative viruses in 112 patients (56.0%), including 16 co-infected cases (8.0%). A total of 129 viruses were identified: respiratory syncytial virus A/B (38.0%/7.8%), influenza virus A/B (10.1%/5.4%), parainfluenza virus 1/2/3 (7.0%/3.1%/7.8%), coronavirus 229E or NL63 (6.2%), human metapneumovirus (4.7%), adenovirus (4.7%), rhinovirus (3.9%), and coronavirus OC43 (1.6%). CONCLUSIONS: DPO-based mRT-PCR was found as a sensitive tool for the detection of the viruses that cause childhood respiratory infections. Clinical significances of the agents detected by mRTPCR need further evaluations.
