Point mutation at codon 12 of the c-Ha-ras gene in human gastric cancers..
10.3346/jkms.1992.7.2.110
- Author:
Eun Hee KOH
1
;
Hyun Cheol CHUNG
;
Kyi Beom LEE
;
Eun Kyung HAN
;
Sang Hwan OH
;
Jin Sik MIN
;
Eui Mook CHOI
;
Jung Koo YOUN
;
Byung Soo KIM
Author Information
1. Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
c-Ha-ras gene;
point mutation;
codon;
polymerase chain reaction
- MeSH:
Base Sequence;
*Codon;
*Genes, ras;
Humans;
Molecular Sequence Data;
*Mutation;
Nucleic Acid Hybridization;
Polymerase Chain Reaction;
Stomach Neoplasms/*genetics
- From:Journal of Korean Medical Science
1992;7(2):110-115
- CountryRepublic of Korea
- Language:English
-
Abstract:
The molecular mechanisms of the carcinogenic process of gastric cancer have not been fully understood yet. In order to know whether c-Ha-ras gene is being involved in the process of gastric carcinogenesis, 8 gastric cancer cell lines, 8 cases of gastric cancer and same number of adjacent dysplasia were analyzed for the presence of mutation at codon 12, 13 and 61 of the c-Ha-ras gene by using polymerase chain reaction (PCR) and mutant-specific oligonucleotide hybridization. Point mutations at codon 12 of the c-Ha-ras gene were found in 2 out of 8 gastric cancer and dysplasia samples in one case, but we found no mutation at codon 13 or 61 of the c-Ha-ras gene. These results suggest that the frequency of mutation of the c-Ha-ras gene detected by sensitive PCR technique is low indeed, however it would be notable that such a genetic change has been detected in the dysplastic lesion of the gastric cancer patient.
