Expression Profiles of F4/80 and Nestin in Ocular Immune Cells Following Pharmaceutically Induced Retinal Degeneration in Adult Mice.
10.3341/jkos.2013.54.6.945
- Author:
Sang Il AHN
1
;
Young Hoon OHN
;
Tae Kwann PARK
Author Information
1. Department of Ophthalmology, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon, Korea. tkpark@schmc.ac.kr
- Publication Type:Original Article
- Keywords:
F4/80;
MNU;
Nestin;
Retinal degeneration
- MeSH:
Adult;
Animals;
Apoptosis;
Ciliary Body;
Coloring Agents;
Eosine Yellowish-(YS);
Eye;
Hematoxylin;
Humans;
Immunohistochemistry;
Injections, Intraperitoneal;
Intermediate Filament Proteins;
Methylnitrosourea;
Mice;
Nerve Tissue Proteins;
Optic Nerve;
Retina;
Retinal Degeneration;
Retinaldehyde
- From:Journal of the Korean Ophthalmological Society
2013;54(6):945-953
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the expression patterns of F4/80 and nestin in the ciliary body and the optic nerve following N-methyl-N-nitrosourea (NMU)-induced retinal degeneration in adult mice. METHODS: After intraperitoneal injection of MNU (60 mg/kg) in adult mice, the eyes were enucleated at 2, 4, 7 and 30 days. Hematoxylin and eosin (H&E) stain, terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stain and immunohistochemical stains of F/80 and nestin were performed. RESULTS: After MNU treatment, the photoreceptors were destroyed by cell apoptosis. According to immunohistochemistry, F4/80 and nestin were not co-expressed in the control group, but F4/80 was expressed within the ciliary body and optic nerve in the MNU-treated group; the expression of nestin also increased. In the outer nuclear layer, F4/80 and nestin co-expressing cells were observed. CONCLUSIONS: In response to retinal damage, the F4/80 and nestin co-expressing cells migrated to the retina from the ciliary body and optic nerve and were activated.
