Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis.
10.3347/kjp.2003.41.2.129
- Author:
Seung Young HWANG
1
;
Hye Jin HAN
;
So Hee KIM
;
Sae Gwang PARK
;
Dae Hyun SEOG
;
Na Ri KIM
;
Jin HAN
;
Joon Yong CHUNG
;
Weon Gyu KHO
Author Information
1. Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Clonorchis sinensis;
potassium channels;
cloning
- MeSH:
Adenosine Triphosphate/metabolism;
Amino Acid Sequence;
Animals;
Base Sequence;
Cloning, Molecular;
Clonorchis sinensis/*genetics/metabolism;
Helminth Proteins/*genetics/metabolism;
Human;
Molecular Sequence Data;
Potassium Channels, Inwardly Rectifying/*genetics/metabolism;
RNA, Helminth/chemistry/genetics;
Sequence Alignment;
Support, Non-U.S. Gov't
- From:The Korean Journal of Parasitology
2003;41(2):129-133
- CountryRepublic of Korea
- Language:English
-
Abstract:
A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-senstive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino acid sequence revealed a highly conserved sequence as other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.
