Synergistic Effect of Parthenolide in Combination with 5-Fluorouracil in SW480 Cells.
- Author:
Se Lim KIM
1
;
Thu Trang Thi KIEU
;
Byung Jun JEON
;
Seong Hun KIM
;
In Hee KIM
;
Seung Ok LEE
;
Soo Teik LEE
;
Sang Wook KIM
Author Information
- Publication Type:Original Article
- Keywords: Parthenolide; 5-Fluorouracil; Apoptosis; Colorectal Neoplasms
- MeSH: Apoptosis; Bisbenzimidazole; Blotting, Western; Caspase 8; Cell Death; Chromatin; Colorectal Neoplasms; DNA; Drug Resistance; Fluorescein-5-isothiocyanate; Fluorouracil; Humans; Sesquiterpenes; Tanacetum parthenium
- From:Intestinal Research 2012;10(4):357-364
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND/AIMS: Parthenolide (PT) is responsible for the bioactivities of Feverfew. Besides its potent anti-inflammatory effect, this compound has recently been reported to induce apoptosis in cancer cells. Unfortunately, many of the therapies that use 5-fluorouracil (5-FU) alone or in combination with other agents are likely to become ineffective due to drug resistance. In the present study, we investigate the antitumor effect of PT combined with 5-FU on colorectal cancer cells. METHODS: SW480 cell was employed as a representative of human colorectal carcinoma (CRC) cells. We performed MTT, annexin-V assay, and Hoechst 33258 staining to measure the synergistic effect. Western blotting was used to demonstrate apoptotic pathway. RESULTS: Our result demonstrated that PT inhibited the viability of colorectal cancer cells and had synergistic anti-proliferation in combination with 5-FU. After combined treatment of 5-FU and PT, enhanced apoptotic cell death is observed using annexin-V FITC assay and it was revealed by the condensed chromatin and fragmented DNA. Compared with 5-FU or PT alone, the apoptosis of colorectal cancer cells treated with PT and 5-FU enhanced the activation of caspase-8, caspase-3. CONCLUSIONS: Combined treatment with PT may offer an efficacious strategy to overcome 5-FU resistance in certain CRC cells.
