A Novel Model for Human Atopic Dermatitis: Application of Repeated DNCB Patch in BALB/c Mice, in Comparison with NC/Nga Mice.
- Author:
Kyoung Sun LEE
1
;
Eui Suk JEONG
;
Seung Ho HEO
;
Jin Hee SEO
;
Dong Gu JEONG
;
Yang Kyu CHOI
Author Information
- Publication Type:Original Article
- Keywords: Atopic dermatitis; BALB/c; 2,4-dinitrochlorobenzene (DNCB); NC/Nga
- MeSH: Animals; Dermatitis, Atopic; Dinitrochlorobenzene; Humans; Hyperplasia; Immunoglobulin E; Lymph Nodes; Mast Cells; Mice; Reproduction; RNA, Messenger
- From:Laboratory Animal Research 2010;26(1):95-102
- CountryRepublic of Korea
- Language:English
- Abstract: The various murine models have contributed to the study of human atopic dermatitis (AD). However limitations of the models involve low reproducibility and long time to develop AD. In an attempt to overcome these limitations and establish an atopic dermatitis murine model, we repeated the application of 2, 4-dinitrochlorobenzene (DNCB) patch in NC/Nga and BALB/c mice, which has advantages in reproduction and cost. For the sensitization, a 1 cm2 gauze-attached patch, where 1% or 0.2% DNCB was periodically attached on the back of NC/Nga and BALB/c mice. To estimate how homologous our model was with human atopic dermatitis, clinical, histological and immunological alterations were evaluated. Both strains showed severe atopic dermatitis, increase in subiliac lymph node weight, mast cells, epidermal hyperplasia and serum IgE levels. Though both exhibited a high IL-4/IFN-gamma and IL-4/TNF-beta ratio in the expression of mRNA, the shifting of DNCB-treated BALB/c mice was increased to more than double that of NC/Nga mice. These results suggest that our DNCB patched model using BALB/c mice were more suitable than NC/Nga mice in demonstrating the immune response. We anticipate that our novel model may be successfully used for pathogenesis of atopic dermatitis and assessment of therapeutic approaches.
