Development and Evaluation of the Quick Anaero-system-A New Disposable Anaerobic Culture System.
10.3343/kjlm.2010.30.2.133
- Author:
Nam Woong YANG
1
;
Jin Man KIM
;
Gwang Ju CHOI
;
Sook Jin JANG
Author Information
1. Department of Microbiology, Chosun University Medical School, Gwangju, Korea.
- Publication Type:Original Article ; Comparative Study ; Evaluation Studies
- Keywords:
Anaerobic bacteria;
Culture techniques;
Culture media
- MeSH:
Bacteria, Anaerobic/*growth &development;
Bacteriological Techniques/instrumentation/methods;
Culture Media/chemistry;
Gases/chemistry;
Palladium/chemistry;
Reagent Kits, Diagnostic
- From:The Korean Journal of Laboratory Medicine
2010;30(2):133-137
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: We developed a new disposable anaerobic culture system, namely, the Quick anaero-system, for easy culturing of obligate anaerobes. METHODS: Our system consists of 3 components: 1) new disposable anaerobic gas pack, 2) disposable culture-envelope and sealer, and 3) reusable stainless plate rack with mesh containing 10 g of palladium catalyst pellets. To evaluate the efficiency of our system, we used 12 anaerobic bacteria. We prepared 2 sets of ten-fold serial dilutions of the 12 anaerobes, and inoculated these samples on Luria-Bertani (LB) broth and LB blood agar plate (LB-BAP) (BD Diagnostic Systems, USA). Each set was incubated in the Quick anaero-system (DAS Tech, Korea) and BBL GasPak jar with BD GasPak EZ Anaerobe Container System (BD Diagnostic Systems) at 35-37degrees C for 48 hr. The minimal inoculum size showing visible growth of 12 anaerobes when incubated in both the systems was compared. RESULTS: The minimal inoculum size showing visible growth for 2 out of the 12 anaerobes in the LB broth and 9 out of the 12 anaerobes on LB-BAP was lower for the Quick anaero-system than in the BD GasPak EZ Anaerobe Container System. The mean time (+/-SD) required to achieve absolute anaerobic conditions of the Quick anaero-system was 17 min and 56 sec (+/-3 min and 25 sec). CONCLUSIONS: The Quick anaero-system is a simple and effective method of culturing obligate anaerobes, and its performance is superior to that of the BD GasPak EZ Anaerobe Container System.
