Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells.
10.3341/jkos.2011.52.9.1089
- Author:
Jeong Hun LEE
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Atropine;
Cyclopentolate;
Pilocarpine;
Trabecular meshwork cells;
Tropicamide
- MeSH:
Atropine;
Constriction;
Cyclopentolate;
Humans;
Nitric Oxide;
Pilocarpine;
Pupil;
Trabecular Meshwork;
Tropicamide
- From:Journal of the Korean Ophthalmological Society
2011;52(9):1089-1093
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of pupil dilation and constriction agents on the survival and production of nitric oxide (NO) in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 0.01, and 0.1 mg/ml of tropicamide, cyclopentolate, atropine, or pilocarpine for 2 hours. Cellular survival and production of NO were assessed using the MTT assay and Griess assay, respectively. RESULTS: Tropicamide, cyclopentolate, atropine, and pilocarpine decreased cellular survival at the concentration of 0.1 mg. At the concentration of 0.01 mg/ml, all agents decreased production of NO to some extent, although the reduction was not statistically significant. CONCLUSIONS: Pupil dilation and constriction agents may be toxic to HTMC if used at high concentrations or if used frequently in the short-term but may not affect trabecular outflow.
