Anti-adenoviral Effects of Human Cationic Antimicrobial Protein-18/LL-37, an Antimicrobial Peptide, by Quantitative Polymerase Chain Reaction.
10.3341/kjo.2013.27.3.199
- Author:
Eiichi UCHIO
1
;
Hirotoshi INOUE
;
Kazuaki KADONOSONO
Author Information
1. Department of Ophthalmology, Fukuoka University School of Medicine, Fukuoka, Japan. euchio@fukuoka-u.ac.jp
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Adenoviridae;
Antiviral agents;
Human cationic antimicrobial protein-18;
LL-37;
Viral conjunctivitis
- MeSH:
Adenocarcinoma;
Adenoviridae/*drug effects/*genetics;
Adenoviridae Infections/*drug therapy/virology;
Antimicrobial Cationic Peptides/*pharmacology;
Cell Line, Tumor;
DNA, Viral/genetics;
Humans;
Keratoconjunctivitis/*drug therapy/virology;
Lung Neoplasms;
Reverse Transcriptase Polymerase Chain Reaction/methods
- From:Korean Journal of Ophthalmology
2013;27(3):199-203
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Antimicrobial peptides have an important role in self-protection of the ocular surface. Human cationic antimicrobial protein (hCAP)-18 is a linear, alpha-helical peptide that consists of a conserved pro-sequence called a cathelin-like domain and a C-terminal peptide named LL-37. We investigated the in vitro anti-adenoviral activity of hCAP-18/LL-37 in several adenovirus types, inducing keratoconjunctivitis. METHODS: A549 cells were used for viral cell culture, and human adenovirus (HAdV) types 3 (HAdV3, species B), 4 (species E), 8, 19a, and 37 (species D) were used. The cytotoxicity of LL-37 was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay to obtain 50% cytotoxic concentration. After pretreatment of A549 cells with serial dilutions of LL-37 for 24 hours, adenovirus was cultured for seven days, and adenoviral DNA was quantitatively measured by real-time polymerase chain reaction (PCR). RESULTS: The 50% effective concentration of LL-37 obtained by real-time PCR ranged between 118 and 270 microM. LL-37 showed a significant inhibitory effect on adenoviral proliferation in all adenovirus types except HAdV4 in a dose-dependent manner. CONCLUSIONS: LL-37 has significant inhibitory activity against HAdV3, 8, and 19, which induce keratoconjunctivitis. These results indicate that hCAP-18/LL-37 may be a possible candidate for the treatment of HAdV keratoconjunctivitis.
