Suppression of MicroRNA let-7a Expression by Agmatine Regulates Neural Stem Cell Differentiation.
10.3349/ymj.2016.57.6.1461
- Author:
Juhyun SONG
1
;
Yumi OH
;
Jong Youl KIM
;
Kyoung Joo CHO
;
Jong Eun LEE
Author Information
1. Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea. jelee@yuhs.ac
- Publication Type:Original Article
- Keywords:
Neural stem cell (NSCs);
microRNA let-7a (let-7a);
agmatine;
DCX;
TLX;
ERK
- MeSH:
Agmatine*;
Biogenic Amines;
Cell Differentiation;
Cell Proliferation;
Central Nervous System;
MicroRNAs*;
Neural Stem Cells*;
Neurogenesis;
Neurons
- From:Yonsei Medical Journal
2016;57(6):1461-1467
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Neural stem cells (NSCs) effectively reverse some severe central nervous system (CNS) disorders, due to their ability to differentiate into neurons. Agmatine, a biogenic amine, has cellular protective effects and contributes to cellular proliferation and differentiation in the CNS. Recent studies have elucidated the function of microRNA let-7a (let-7a) as a regulator of cell differentiation with roles in regulating genes associated with CNS neurogenesis. MATERIALS AND METHODS: This study aimed to investigate whether agmatine modulates the expression of crucial regulators of NSC differentiation including DCX, TLX, c-Myc, and ERK by controlling let-7a expression. RESULTS: Our data suggest that high levels of let-7a promoted the expression of TLX and c-Myc, as well as repressed DCX and ERK expression. In addition, agmatine attenuated expression of TLX and increased expression of ERK by negatively regulating let-7a. CONCLUSION: Our study therefore enhances the present understanding of the therapeutic potential of NSCs in CNS disorders.
