Coptis chinensis Extract Inhibits the Production of Inflammatory Mediators and Delayed Type Hypersensitivity in Mice.
- Author:
Yeon Ah LEE
1
;
Seung Jae HONG
;
Sang Hoon LEE
;
Kyoung Soo KIM
;
Eun Kyung PARK
;
Kiwon JUNG
;
Chung Soo HAN
;
Myung Chul YOO
;
Hyung In YANG
Author Information
- Publication Type:Original Article
- Keywords: Coptis chinensis; inflammatory cytokine; inflammatory mediator; delayed type hypersensitivity (DTH)
- MeSH: Animals; Cell Proliferation; Coptis; Enzyme-Linked Immunosorbent Assay; Ethanol; Hypersensitivity; Inflammation; Interleukins; Macrophages; Medicine, East Asian Traditional; Mice; Nitric Oxide; Plants, Medicinal; Rhizome
- From:Immune Network 2008;8(1):13-20
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Coptis chinensis rhizome has been used as a medicinal herb in traditional Oriental medicine. We investigated the effects of Coptis chinensis extract on inflammatory mediators and delayed type hypersensitivity in mice. METHODS: The inhibitory effect of ethanolic extract of Coptis chinensis (CCE) on cell proliferation was evaluated using MTS assay. The lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and the Con A-activated mouse splenocytes were cultured with various concentrations of CCE. Total nitric oxide (NO) production was determined by Griess reaction. The amounts of secreted prostaglandine E2 (PGE(2)), interleukin (IL)-2 and IFN-gamma were measured by ELISA. To investigate the in vivo anti-inflammatory effect of CCE, oxazolone-induced delayed type hypersensitivity (DTH) model was used. RESULTS: The CCE at 100 microgram/ml significantly blocked the LPS-induced production of pro-inflammatory mediators (NO and PGE) in RAW264.7 macrophages. Also, it significantly inhibited cell proliferation and cytokine (IL-2 and IFN-gamma) production in splenocytes. Furthermore, when splenocytes from CCE fed mice (200 mg/kg for 2 weeks) were activated with Con A, cell proliferation and cytokine production were significantly inhibited. In addition, CCE decreased in vivo inflammation in oxazolone-induced DTH model mice. CONCLUSION: We suggest that Coptis chinensis can be used as an anti-inflammatory drug by exerting an inhibitory effect in inflammatory mediator- and cell-mediated inflammation.
