In Vitro Adenosine Triphosphate Based Chemotherapy Response Assay in Gastric Cancer.
10.5230/jgc.2010.10.4.155
- Author:
Seulkee PARK
1
;
Yanghee WOO
;
Hogeun KIM
;
Yong Chan LEE
;
Sungho CHOI
;
Woo Jin HYUNG
;
Sung Hoon NOH
Author Information
1. Department of Surgery, Yonsei University College of Medicine, Seoul, Korea. wjhyung@yuhc.ac
- Publication Type:In Vitro ; Original Article
- Keywords:
Stomach neoplasms;
Chemosensitivity assay;
ATP based chemoresponse
- MeSH:
Adenosine;
Adenosine Triphosphate;
Camptothecin;
Cell Death;
Cisplatin;
Doxorubicin;
Epirubicin;
Etoposide;
Fluorouracil;
Gastrectomy;
Humans;
Lymph Nodes;
Methotrexate;
Organoplatinum Compounds;
Paclitaxel;
Polyphosphates;
Stomach Neoplasms;
Taxoids
- From:Journal of Gastric Cancer
2010;10(4):155-161
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The purpose of this study was to investigate the reliability and the clinical applicability of the adenosine-triphosphate-based chemotherapy response assay (ATP-CRA) as a method of determining in vitro chemosensitivity in patients with gastric cancer. MATERIALS AND METHODS: A total of 243 gastric cancer tissue samples were obtained from gastrectomies performed between February 2007 and January 2010. We evaluated the effectiveness of the ATP-CRA assay in determining the chemosensitivity of gastric cancer specimens using eleven chemotherapeutic agents - etoposide, doxorubicin, epirubicin, mytomicin, 5-fluorouracil, oxaliplatin, irinotecan, docetaxel, paclitaxel, methotraxate, and cisplatin - for chemosensitivity studies using ATP-CRA. We assessed the failure rate, the cell death rate, and the chemosensitivity index. RESULTS: The failure rate of ATP-CRA was 1.6% (4/243). The mean coefficient of variation for triplicate ATP measurements was 6.5%. Etoposide showed the highest cell death rate (35.9%) while methotrexate showed the lowest (16.6%). The most active chemotherapeutic agent was etoposide, which most frequently ranked highest in the chemosensitivity test: 31.9% (51/160). Oxaliplatin was more active against early gastric cancers than advanced gastric cancers, whereas docetaxel was more active against advanced cancers. The lymph node negative group showed a significantly higher cell death rate than the lymph node positive group when treated with doxorubicin, epirubicin, and mitomycin. CONCLUSIONS: ATP-CRA is a stable and clinically applicable in vitro chemosensitivity test with a low failure rate. The clinical usefulness of ATP-CRA should be evaluated by prospective studies comparing the regimen guided by ATP-CRA with an empirical regimen.
