VEGF-related Autocrine Growth in Periosteal-Derived Cells.
- Author:
Bong Wook PARK
1
;
Seong Gyun LEE
;
Young Sool HAH
;
Deok Ryong KIM
;
Yeong Cheol CHO
;
Iel Yong SUNG
;
Uk Kyu KIM
;
Jong Ryoul KIM
;
June Ho BYUN
Author Information
1. Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21), Korea. surbyun@gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
Periosteal-derived cells;
Vascular endothelial growth factor;
Autocrine growth
- MeSH:
Alkaline Phosphatase;
Ascorbic Acid;
Calcium;
Cell Culture Techniques;
Dexamethasone;
Durapatite;
Glycosaminoglycans;
Homeostasis;
Humans;
Osteoblasts;
Periosteum;
Phosphotransferases;
Protein Isoforms;
Vascular Endothelial Growth Factor A;
Vascular Endothelial Growth Factor Receptor-1;
Vascular Endothelial Growth Factor Receptor-2
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2009;35(5):294-298
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells. MATERIALS AND METHODS: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. RESULTS: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. CONCLUSION: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
