Enhancement of the Stability and Skin Penetration of Vitamin C by Polyphenol.
- Author:
Jae Seung KANG
1
;
Daeho CHO
;
Wang Jae LEE
Author Information
- Publication Type:Original Article
- Keywords: Vitamin C; polyphenol; histamine; atopic dermatitis
- MeSH: Ascorbic Acid*; Asthma; Chromatography, High Pressure Liquid; Collagen; Dermatitis, Atopic; Diet; Evans Blue; Histamine; Humans; Hypersensitivity; Mast Cells; Scurvy; Skin*; Vitamins*
- From:Immune Network 2004;4(4):250-254
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: It is necessary for human beings to uptake vitamin C through diet or supplements. It is also well-known that vitamin C plays an important role in the prevention of scurvy, enhancement of collagen synthesis and anti-tumor immune response. In addition, there are several recent reports regarding the effective role of vitamin C on the regulation of allergic responses, such as atopic dermatitis and asthma. However, the effective therapeutic and preventive measures using vitamin C are not established yet, since vitamin C is seriously unstable in aqueous solution. Therefore, we have investigated the best way to maintain the stability of vitamin C. METHODS: After we making a mixture of polyphenol (0.001, 0.01, 0.1%) and vitamin C (1 mM), the mixtures were placed at room temperature both with/without light protection. And then the concentration of ascorbic acid was measured with HPLC. To analyze the in vivo effect of vitamin C on the regulation of skin allergic reaction, polyphenol (0.1%)-vitamin C (1 mM) mixture was applied to the skin and the production of histamine from mast cell was analyzed by Evans blue dye staining. RESULTS: We have found that the polyphenol has preventive power of oxidation of vitamin C. In addition, the production of histamine was suppressed by the polyphenol (0.1%)-vitamin C (1 mM) mixture. CONCLUSION: We have reached the conclusion that our study suggests the research guideline for the therapy of atopic dermatitis through vitamin C.
