The Effect of Human Umbilical Cord Blood Derived Mesenchymal Stem Cell Therapy in Rat Model of Cavernosal Nerve Injury.
- Author:
Aram CHOI
1
;
Sung Dae KIM
;
Dong Wan SOHN
;
Doo Bae KIM
;
Hyun Woo KIM
;
Su Yeon CHO
;
Yong Hyun CHO
;
Sae Woong KIM
Author Information
1. Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ksw1227@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Erection;
Corpus cavernosum;
Human mesenchymal stem cells
- MeSH:
Animals;
Arterial Pressure;
Fetal Blood;
Ganglion Cysts;
Humans;
Male;
Mesenchymal Stromal Cells;
Nerve Crush;
Organic Chemicals;
Rats;
Rats, Sprague-Dawley;
Salicylamides;
Transplants;
Umbilical Cord
- From:Korean Journal of Andrology
2008;26(3):136-141
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To find out the effect of transplanted umbilical cord blood derived human mesenchymal stem cells (hUCB- MSCs) in the rat model of bilateral cavernosal nerve injury (BCNX) MATERIALS AND METHODS: Male Sprague Dawley rats were divided into three groups: Group I (7 rats, Control, Sham operation) had only bilateral cavernosal nerve crushing injury. PKH-26 fluorescent cell linker was labeled to hUCB- MSCs Group II (7 rats) had transplantation of hUCB- MSCs (3x10(5)cells/50microliter PBS) into MPG after BCNX. Group III (7 rats) had transplantation of hUCB- MSCs (3x10(5)cells/50microliter PBS) into the CC after BCNX. We detected the PKH-26 labeled MSCs in Group II, and III at four weeks after transplantation. Peak intracavernosal pressure (ICP) and ICP/mean arterial pressure (MAP) were recorded after pelvic nerve stimulation at 12 weeks after transplantation. RESULTS: PKH-26 labeled hUCB-MSCs were detected in MPG and CC of group II and III at four weeks after transplantation. The peak ICP was significantly higher in the Group II (69.6+/-5.9mmHg) and Group III (59.7+/-7.6mmHg) compared to Group I40.6+/-10.5mmHg) at 12 weeks after transplantation. The rates of ICP/MAP were significantly higher in Group II (32.2+/-6.4%) and Group III (57.8+/-4.9%) compared to Group I (49.5+/-2.9%) at 12 weeks after transplantation. CONCLUSIONS: The intracavernosal and major pelvic ganglion transplantation of hUCB- MSCs improve erectile function in a rat model of neurogenic impotence.
