Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry.
- Author:
Jia KIM
1
;
Young Sang SONG
;
Kyung San MIN
;
Sun Hun KIM
;
Jeong Tae KOH
;
Bin Na LEE
;
Hoon Sang CHANG
;
In Nam HWANG
;
Won Mann OH
;
Yun Chan HWANG
Author Information
- Publication Type:Original Article
- Keywords: BioAggregate; Biodentine; Dentin sialoprotein; Micro-CT; MTA
- MeSH: Animals; Calcium; Dentin*; Immunohistochemistry*; Rats; Pemetrexed
- From:Restorative Dentistry & Endodontics 2016;41(1):29-36
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). MATERIALS AND METHODS: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP). RESULTS: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP. CONCLUSIONS: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.
