The Prevalence of Transfusion-Transmitted Virus Infection in Children.
- Author:
Ju Young CHUNG
1
;
Tae Hee HAN
;
Eung Su HWANG
Author Information
1. Department of Pediatrics, College of Medicine, Inje University, Seoul, Korea. pedchung@sanngyepaik.ac.kr
- Publication Type:Original Article
- Keywords:
Transfusion-Transmitted virus;
Children
- MeSH:
Child*;
DNA;
Genome;
Genome, Viral;
Genotype;
Hepatitis;
Humans;
Liver Diseases;
Polymerase Chain Reaction;
Prevalence*;
Torque teno virus*
- From:Korean Journal of Pediatrics
2004;47(2):183-186
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Transfusion-transmitted virus(TTV) is an newly described nonenveloped human virus, with a circular, negative stranded DNA genome. Although a high prevalence of TTV infection in the normal population has been demonstrated, there is a still possibility of association with hepatitis according to the genotype of TTV. The aim of this study is to investigate the prevalence of TTV infection in Korean children. METHODS: Nested polymerase chain reaction(PCR) using priner sets generated from the noncoding region(NCR) of the viral genome was done in 105 children without liver disease, aged 0-15 years. We performed a second set of PCR using N22 primer in 88 children after the first set of PCR. RESULTS: The TTV DNA was detectable in 36(34%) of 105 children without hepatitis by 5'NCR primer. The prevalence of TTV varied with age:<1 y,16%(4/25); 1-3 y, 44%(15/31); 4-6 y, 31%(5/ 16); 7-9 y, 25%(3/12); 10-15 y, 14%(3/21). By using N22 primers, the prevalence of TTV DNA in children without hepatitis was 11.3%(11/88):<1 y 8%(2/25); 1-3 y, 13.7%(4/29); 4-6 y, 6.2%(1/16); 7-9 y, 33.3%(2/6); 10-14 y, 8.2%(1/12). CONCLUSION: Our result showed a high prevalence of TTV infection, varying with age, in Korean children. Further evaluation of genotypes of TTV in patients with hepatitis and normal children is needed.
