Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
- Author:
Ji Ye AHN
1
;
Il Yong KIM
;
Sae Jin OH
;
Hye Sook HWANG
;
Sun Shin YI
;
Yo Na KIM
;
Jae Hoon SHIN
;
Yeo Sung YOON
;
Je Kyung SEONG
Author Information
- Publication Type:Original Article
- Keywords: Domestic pig; pancreas; development; 2-DE proteomics; MALDI-TOF
- MeSH: Adult; alpha-Amylases; alpha-Fetoproteins; Chymotrypsin; Electrophoresis*; Electrophoresis, Gel, Two-Dimensional; Humans; Infant, Newborn; Lipase; Mass Spectrometry*; Pancreas*; Parturition; Pregnancy; Sus scrofa*; Transferrin; Transferrins; Trypsin
- From:Laboratory Animal Research 2014;30(2):45-53
- CountryRepublic of Korea
- Language:English
- Abstract: Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential is hindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, the embryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5 protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerol lipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressed in neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alpha-fetoprotein and transferrins. Taken together, we found out that several proteins were significantly up- or down- regulated from pig pancreas based on developmental stage. This study will provide basis for understanding development of pig pancreas.
