A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis.
- Author:
Seong Min PARK
1
;
Sung Joon PARK
;
Hee Jin KIM
;
Oh Hyung KWON
;
Tae Wook KANG
;
Hyun Ahm SOHN
;
Seon Kyu KIM
;
Seung MOO NOH
;
Kyu Sang SONG
;
Se Jin JANG
;
Yong SUNG KIM
;
Seon Young KIM
Author Information
1. Medical Genomics Research Center, KRIBB, Daejeon, Republic of Korea. kimsy@kribb.re.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
gastric cancer;
high-throughput RNA sequencing;
long intergenic non-coding RNA;
non-coding RNA;
RNA-seq
- MeSH:
Animals;
DNA, Intergenic/genetics;
Expressed Sequence Tags/*metabolism;
Extracellular Space/metabolism;
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic;
Genetic Predisposition to Disease;
Humans;
Male;
Matrix Metalloproteinase 9/metabolism;
Mice;
Mice, Inbred C57BL;
Multivariate Analysis;
Neoplasm Metastasis;
Neoplasm Staging;
Phenotype;
Proportional Hazards Models;
RNA, Long Noncoding/*genetics/metabolism;
RNA, Messenger/genetics/metabolism;
Reproducibility of Results;
Stomach Neoplasms/*genetics/*pathology;
Survival Analysis
- From:Experimental & Molecular Medicine
2013;45(7):e31-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Long intergenic non-coding RNAs (lincRNAs) have historically been ignored in cancer biology. However, thousands of lincRNAs have been identified in mammals using recently developed genomic tools, including microarray and high-throughput RNA sequencing (RNA-seq). Several of the lincRNAs identified have been well characterized for their functions in carcinogenesis. Here we performed RNA-seq experiments comparing gastric cancer with normal tissues to find differentially expressed transcripts in intergenic regions. By analyzing our own RNA-seq and public microarray data, we identified 31 transcripts, including a known expressed sequence tag, BM742401. BM742401 was downregulated in cancer, and its downregulation was associated with poor survival in gastric cancer patients. Ectopic overexpression of BM742401 inhibited metastasis-related phenotypes and decreased the concentration of extracellular MMP9. These results suggest that BM742401 is a potential lincRNA marker and therapeutic target.
