Differentially expressed proteins in the liver of Gulo-/- mice following treatments with Helicobacter pylori and diethylnitrosamine.
- Author:
Arulkumar NAGAPPAN
1
;
Hyeon Soo PARK
;
Kwang Il PARK
;
Jin A KIM
;
Gyeong Eun HONG
;
Silvia YUMNAM
;
Eun Hee KIM
;
Won Sup LEE
;
Wang Jae LEE
;
Myung Je CHO
;
Woo Kon LEE
;
Chung Kil WON
;
Gon Sup KIM
Author Information
1. Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea. gonskim@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Gulo-/- mice;
helicobacter pylori;
diethylnitrosamine;
proteome analysis
- MeSH:
Animals;
Ascorbic Acid;
Avitaminosis;
Diet;
Diethylnitrosamine*;
Helicobacter pylori*;
Helicobacter*;
Humans;
Immunohistochemistry;
Liver*;
Mice*;
Oxidative Stress;
Proteins*;
Proteome
- From:Journal of Biomedical Research
2013;14(2):99-104
- CountryRepublic of Korea
- Language:English
-
Abstract:
Vitamin C (ascorbic acid) is an essential nutrient of most living tissues. We established a strain of Gulo-/- mice with known deficiency, in which vitamin C intake can be controlled by diet, like humans, and investigated the differentially expressed proteins following treatments with Helicobacter pylori and diethylnitrosamine (DENA) in the liver of Gulo-/- mice using a proteomic approach. Expression of p53, 14-3-3epsilon and 14-3-3delta in Gulo-/- mice liver tissue was analyzed by immunohistochemistry. 2-DE maps constructed from Gulo-/- mice liver and differentially expressed proteins in liver tissue were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS). In Gulo-/- mice after H. Pylori infection, followed by treatment with DENA, no differences in p53, 14-3-3epsilon and 14-3-3delta were observed by immunohistochemistry. Proteome analyses using MALDI-TOF/MS resulted in successful identification of 12 proteins (nine proteins were up-regulated and three were down-regulated). Specifically, peroxiredoxin-6 and Alpha-1-antitrypsin 1-4 were up-regulated in liver after H. Pylori infection followed by treatment with DENA. These results indicated that oral supplementation with vitamin C led to rescue of Gulo-/- mice from vitamin deficiency, and protected the liver from H.pylori infection and/or DENA effect, and vitamin C also protected the liver against oxidative stress.
