Results of a study on the effect of Notch signaling pathway inhibitors on ameloblastoma cell proliferation
- VernacularTitle:Notch дохио дамжих замын дарангуйлагчийн амелобластомагийн эсийн ургалтад үзүүлэх нөлөөг судалсан дүн
- Author:
Baoyinbatu
1
;
2
;
Wurihan
2
;
3
;
Baolidao
2
;
Damdindorj B
1
;
Sarnai Ts
1
Author Information
1. Graduate School of MNUMS
2. Affiliated Hospital of Chifeng University, Inner Mongolia, China
3. Department of Obstetrics and Gynecology, School of Medicine, MNUMS
- Publication Type:Journal Article
- Keywords:
Notch1, Cyclin D1, CDK1, FLI-06.
- From:
Mongolian Journal of Health Sciences
2025;90(6):184-188
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Ameloblastoma (AB) is an odontogenic epithelial tumor. 80% of ameloblastomas occur in the jaw and
often painlessly enlarge the jaw and cause facial deformity. Ameloblastoma is usually treated with a complete osteotomy,
which is divided into three types: partial maxillary marginal resection, maxillary sinus resection, and total maxillary
resection. These surgical procedures cause jaw deformity and facial trauma, so effective treatment methods with minimal
trauma are still being sought in medicine. In order to develop new drugs for the treatment of ameloblastoma, it is
necessary to study the molecular mechanisms that inhibit the proliferation of cancer cells. In recent years, it has been
established that ameloblastoma is caused by abnormal activation of signaling pathways. Multiple signaling pathway regulators,
target genes, and molecular interactions are involved in tumor cell proliferation and apoptosis. Previous studies
have shown that blocking key signaling pathways is not only a therapeutic option in clinical practice, but also a useful tool
in selecting tumor resection sites. Therefore, studying the molecular level of the Notch signaling pathway in the proliferation
of ameloblastoma cells is of great importance for the treatment of the disease. Studies have shown that many factors
of the Notch signaling pathway play important roles in promoting or inhibiting different tumors. The Notch signaling
pathway promotes tumor cell proliferation by regulating CDK1, Cyclin D1, HES-1, and COX-2. Chinese researchers Li
Wenchao and Baolidao’s research team have studied factors related to AB proliferation and invasion for the past decade,
and have identified the roles of factors such as COX-2, Survivin, Akt, and PI3K in AB proliferation and invasion, and
have successfully cultured primary ameloblastoma cells. However, the exact role of the Notch signaling pathway in AB
cell proliferation has not been clearly studied.
Aim:To study the effect of Notch signaling pathway inhibitors on ameloblastoma cell proliferation.
Materials and Methods:The study was conducted at the Experimental Research Base of Affiliated Hospital of Chifeng
University, Inner Mongolia, China, using quantitative research methods and experimental research designs. The expression
of Notch1, Cyclin D1, and CDK1 genes in AB cells was detected using real-time polymerase chain reaction. The
effect of FLI-06 on the proliferation of AB cells after inhibition was studied by counting (CCK8) method. The ameloblastoma
cell lines used in the study were obtained from the Experimental Research Base of Affiliated Hospital of Chifeng
University. The Notch signaling pathway inhibitor FLI-06 was purchased from Absin Bioscience Inc. (Absin® CAS:
313967-18-9), Shanghai.
Results:In the study, real-time polymerase chain reaction analysis showed that the expression of Cyclin D1 in AB cells
was 7.01 times higher than that in normal oral mucosa cells, the expression of CDK1 was 2.63 times higher, and the
expression of Notch1 was 4.95 times higher. The cell proliferation of the control group was calculated as 100% by the
AB cell proliferation inhibitor (CCK8) method, and compared with the experimental group, the cell proliferation of the
group added with 5.0μM FLI-06 was 53.98%, 46.53% in the 10.0μM concentration group, and 22.33% in the 20.0μM
concentration group.
Conclusion:Notch1, Cyclin D1, and CDK1 expression in ameloblastoma were significantly different from that in normal
oral mucosa cells. Inhibition of the Notch signaling pathway with FLI-06 reduced ameloblastoma cell proliferation.
- Full text:2025121015274505166Notch дохио дамжих замын дарангуйлагчийн амелобластомагийн эсийн ургалтад үзүүлэх нөлөөг судалсан дүн.pdf
