Effect Mechanism of Guizhi Fulingwan in Regulating Sex Hormone Disorders in Rats with Benign Prostatic Hyperplasia Based on Serum Metabolomics
10.13422/j.cnki.syfjx.20251108
- VernacularTitle:基于血清代谢组学研究桂枝茯苓丸调节良性前列腺增生大鼠性激素紊乱的效应机制
- Author:
Chengchen LI
1
;
Yuanpeng HUANG
2
;
Qian ZHANG
3
;
Dian ZENG
4
;
Lingang KONG
3
;
Yukun FAN
5
;
Yuanduo XIA
3
;
Hao CHEN
6
;
Feng WANG
6
Author Information
1. Third Affiliated Hospital， Beijing University of Chinese Medicine， Beijing 100029， China
2. Xiamen Hospital， Beijing University of Chinese Medicine， Xiamen 361001， China
3. Heilongjiang University of Chinese Medicine， Harbin 150040， China
4. Xiamen Hospital of Traditional Chinese Medicine（TCM）， Fujian University of TCM， Xiamen 361001， China
5. Doumen Hospital， Guangdong Provincial Hospital of TCM， Zhuhai 519100， China
6. Heilongjiang Academy of TCM， Harbin 150040， China
- Publication Type:Journal Article
- Keywords:
Guizhi Fulingwan;
benign prostatic hyperplasia;
serum metabolomics;
sex hormone;
disorder
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(24):21-29
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyse the efficacy and mechanism of Guizhi Fulingwan in regulating sex hormone disorders in rats with benign prostatic hyperplasia （BPH）. MethodsThirty male SD rats were randomly divided into a sham group， a model group， a finasteride group （0.45 mg·kg-1·d-1）， and low-dose and high-dose groups of Guizhi Fulingwan （0.135， 0.337 5 g∙kg-1∙d-1）， with six in each group. The BPH model was prepared by subcutaneous injection of 3.5 mg∙kg-1∙d-1 testosterone propionate after debridement surgery in all groups except the sham group. The rats in the sham group and the model group were administered with an equal volume of saline by gavage， and the rest of the groups were administered with the corresponding medicinal solution by gavage for 35 days. Histopathology in rats was evaluated by prostate wet weight， volume， index， and hematoxylin-eosin （HE） staining. The serum sex hormone levels of testosterone （T）， dihydrotestosterone （DHT）， and estradiol （E2） were determined by enzyme-linked immunosorbent assay. The protein expression of the androgen receptor （AR） was detected by immunohistochemistry. The serum metabolism profiles of rats in the sham group， the model group， and the high-dose group of Guizhi Fulingwan were compared by ultra-high performance liquid chromatography tandem Fourier transform mass spectrometry （UHPLCQ Exactive） to screen for metabolic markers and to obtain relevant metabolic pathways. ResultsCompared with those in the sham group， the wet weight， volume， index， serum sex hormone level， and AR protein expression of the prostate in the model group were all elevated （P<0.05， P<0.01）， and the histomorphology showed pathological changes. Compared with those in the model group， the wet weight， volume， index， serum sex hormone level， and AR protein expression of the prostate in the intervention groups showed a decreasing trend （P<0.05， P<0.01）， and histopathology was improved. Serum metabolomics analysis obtained a total of 40 metabolic markers related to the intervention effect of Guizhi Fulingwan， such as dehydrosafynol， hyoscyamine， and lumichrome， which were involved in the pathways of autophagy， riboflavin metabolism， and retrograde endocannabinoid signaling. ConclusionGuizhi Fulingwan can effectively regulate sex hormone disorders in BPH rats， and its mechanism may be related to autophagy， riboflavin metabolism， and retrograde endocannabinoid signaling.
