Genetic characteristics of VP1 region of Coxsackievirus A10 in Yunnan Province
10.3969/j.issn.1006-2483.2025.06.006
- VernacularTitle:2021—2022年云南省柯萨奇病毒A10病毒VP1基因特征分析
- Author:
Yihui CAO
1
;
Bingjun TIAN
2
;
Zhichao WANG
2
;
Jianping CUN
2
;
Xiaofang ZHOU
2
;
Lili JIANG
2
;
Enfa QIAO
1
Author Information
1. Yunnan Provincial Key Laboratory of Public Health and Biosafety , Kunming ,Yunnan 650500 , China;Yunnan Center for Disease Control and Prevention , Kunming ,Yunnan 650022 , China
2. Yunnan Center for Disease Control and Prevention , Kunming ,Yunnan 650022 , China
- Publication Type:Journal Article
- Keywords:
HFMD;
Enterovirus CVA10;
VP1 gene;
Phylogenetic analysis
- From:
Journal of Public Health and Preventive Medicine
2025;36(6):25-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic characteristics of the VP1 region of Coxsackievirus A10 (CVA10) in Yunnan Province. Methods Fecal samples of suspected hand, foot and mouth disease (HFMD) were subjected to real-time fluorescent quantitative PCR for detection of enterovirus CVA10. Positive samples were subjected to VP1 gene sequence amplification and Sanger sequencing. Sequence splicing was performed with DNAstar7.1 Seqman software, and nucleotide sequence and amino acid site analysis were performed using Mega 6.0 software. Results The sequencing of VP1 gene of CVA10 obtained a sequence of 894 nucleotides, encoding 298 amino acids. Compared with the original strain, there were mainly three active amino acid mutation regions, 13-33, 141-142, and 283-285. The nucleotide difference rate between the Yunnan isolates and the reference strain ranged from 16.92% to 30.90%, and the amino acid difference rate ranged from 2.58% to 4.00%. C1 and C2 group nucleotide difference was 10.58%, and the amino acid difference rate was 1.80%. The VP1 150-176 region exhibited highly conserved characteristics. Six CVA10 strains and Sichuan strain MW178898 belonged to the C1 group of the C genotype. The other 14 CVA10 strains belonged to the C2 group. Conclusion VP1 gene mutation is active and CVA10 is an important pathogen of HFMD in Yunnan. C2 genotype of CVA10 is dominant in this study, and C1 and C2 have co-circulated in Yunnan. It is necessary to strengthen monitoring and develop multivalent vaccines containing CVA10 epidemic genotype.
