Study on the anti-hepatitis mechanism of Abrus pulchellus subsp. cantoniensis （Hance） Verdc. and Abrus pulchellus subsp. mollis （Hance） Verdc. based on serum pharmacochemistry and network pharmacology
- VernacularTitle:基于血清药物化学与网络药理学的鸡骨草和毛鸡骨草抗肝炎作用机制研究
- Author:
Qiaowen ZHOU
1
;
Xue WANG
1
;
Mingjuan HUANG
1
;
Li LI
1
;
Wenya CHEN
1
;
Zhengtao WANG
2
;
Zijia ZHANG
2
;
Lanlan FAN
1
Author Information
1. College of Pharmacy，Guangxi University of Chinese Medicine，Nanning 530200，China
2. Shanghai Key Laboratory of Compound Chinese Medicines，Institute of Chinese Materia Medica，Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China
- Publication Type:Journal Article
- Keywords:
Abrus pulchellus subsp. cantoniensis （Hance） Verdc.;
Abrus pulchellus subsp. mollis （Hance） Verdc.;
anti-hepatitis
- From:
China Pharmacy
2025;36(22):2772-2777
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare the anti-hepatitis mechanisms of Abrus pulchellus subsp. cantoniensis （Hance） Verdc. （AC） and Abrus pulchellus subsp. mollis（Hance） Verdc. （AM）. METHODS SD rats were randomly divided into blank group， AC- treated group， and AM-treated group， with each group consisting of 10 rats. The rats’ orbital venous blood was collected at 5， 15， 30 minutes， and 1， 1.5， 2， 4， 6， 8， 12 hours after gavage administration of 24 g/kg of the corresponding drug （calculated by crude drug） or water， respectively. Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry technology was utilized to identify the prototype components present in the serum. The network pharmacology method was adopted to predict the anti-hepatitis active components， key targets， and signaling pathways of AC and AM. Additionally， molecular docking technology was utilized to verify the binding activity of the core active components with key targets. RESULTS A total of 35 prototype components migrating to the blood of AC and AM were identified in the serum of administered rats， among which 24 were common components. The active components in AC， such as acetylanguidine， physcion， soyasaponin A3 and soyasaponin Ⅰ， as well as those in AM， including vicenin 3， acetylanguidine，soyasaponin Ⅰ and schaftoside， all acted on key targets such as steroid receptor coactivator， phosphatidylinositol-4，5-bisphosphate 3-kinase catalytic subunit alpha， epidermal growth factor receptor （EGFR）， and protein kinase B1（Akt1）. These components modulated pathways in cancer， EGFR tyrosine kinase inhibitor resistance， and the phosphoinositide 3-kinase （PI3K） -Akt pathway， thereby exerting anti-hepatitis effects. Furthermore， the binding energies between these active components and their key targets were all less than －5 kJ/mol. CONCLUSIONS There are differences in the active components of AC and AM against hepatitis， but their mechanisms of action are similar. Both may exert their anti-hepatitis effects through pathways in cancer， EGFR tyrosine kinase inhibitor resistance， and the PI3K-Akt pathway.
