Effects of low-dose fractionated X-ray radiation on the senescence of L02 hepatocytes
10.13491/j.issn.1004-714X.2025.05.009
- VernacularTitle:较低剂量X射线分次辐射对L02肝细胞衰老作用影响
- Author:
Xin LAN
1
;
Lina CAI
2
;
Lingyu ZHANG
3
;
Yashi CAI
3
;
Linqian ZHOU
3
;
Weiyi KE
3
;
Weixu HUANG
3
;
Jianming ZOU
3
;
Huifeng CHEN
1
Author Information
1. Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China;School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
2. Hunan Agricultural University Hospital, Changsha 410125, China.
3. Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China.
- Publication Type:OriginalArticles
- Keywords:
Ionizing radiation;
Hepatocyte;
Senescence;
Oxidative stress;
Nuclear factor-κB
- From:
Chinese Journal of Radiological Health
2025;34(5):672-678
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the induction of senescence in L02 hepatocytes by low-dose fractionated X-ray radiation and its effects on oxidative stress, oxidative damage, and nuclear factor-κB (NF-κB) pathway protein levels. Methods L02 cells were subjected to fractionated X-ray irradiation at doses of 0.1, 0.2, and 0.5 Gy per fraction for a total of six fractions. Assays were performed 24 hours after the final irradiation. Measurements included SA-β-gal staining, the mRNAs of senescence-related genes p53 and p21 and their encoded proteins, mRNAs of genes encoding senescence-associated secretory phenotype factors (IL-6, IL-8, GM-CSF, MMP-15), reactive oxygen species, oxidative and anti-oxidative markers (malondialdehyde, glutathione, superoxide dismutase), DNA oxidative damage markers (8-OHdG and γ-H2AX), and NF-κB pathway protein levels. Results Compared with the control group, at 24 hours after the end of six irradiations, the number of cells positive in SA-β-gal staining was significantly increased in all dose groups. The mRNA and protein levels of p21 and p53 were significantly elevated in the 0.2 Gy × 6 and 0.5 Gy × 6 groups (P < 0.05). The mRNA levels of genes encoding IL-6, GM-CSF, and MMP-15 were significantly increased in all dose groups (P < 0.05). The mRNA levels of the gene encoding IL-8 were significantly increased in the 0.2 Gy × 6 and 0.5 Gy × 6 groups (P < 0.05). The levels of reactive oxygen species, malondialdehyde, and glutathione were significantly increased in all dose groups (P < 0.01). The level of superoxide dismutase was significantly increased in the 0.5 Gy × 6 group (P < 0.01). The levels of 8-OHdG were significantly increased in all dose groups (P < 0.05). In both the 0.2 Gy × 6 and 0.5 Gy × 6 groups, the expression levels of γ-H2AX and p-NF-κB p65 were significantly increased (P < 0.05), and the levels of IκBα were significantly decreased (P < 0.05). Conclusion Low-dose fractionated X-ray radiation can induce senescence and cause alterations in oxidative stress, oxidative damage, and the levels of NF-κB pathway proteins in L02 hepatocytes.
