Pathogenic genetic characteristics of Vibrio vulnificus in commercial foods in Pudong New Area of Shanghai
- Author:
LIANG Yanru
;
LYU Bin
;
HU Yihuan
- Publication Type:Journal Article
- Keywords:
Vibrio vulnificus;
drug resistance;
virulence gene;
resistance gene;
phylogenetic analysis;
commercial food
- From:
China Tropical Medicine
2025;25(1):87-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the contamination status, drug resistance, virulence, genotyping characteristics, and genetic evolution relationships of Vibrio vulnificus in commercially available food in Pudong New Area of Shanghai. Methods The drug susceptibility test was carried out by microbroth dilution method. The whole genome sequencing was used by illumina sequencing platform. The virulence, drug resistance genes and MLST typing were obtained by VFDB, CARD and PubMLST database. Phylogenetic analysis was used by genome-wide single nucleotide polymorphisms. Results The detection rate of Vibrio vulnusis in commercially available food was 4.56% (51/1 119) in 2023, and the dominant detection categories were shellfish（20.16%，25/124）, fish（6.74%，19/282）, shrimp（6.56%，4/61）, ready-to-eat aquatic products（9.09%，2/22） and animal meat（0.33%，1/300）. The highest drug resistance rate of Vibrio vulnificus to cefazolin is 23.5%(12/51), followed by ampicillin, tetracycline, and trimethoprim sulfamethoxazole. And 51 strains of Vibrio vulnificus carried 29 kinds of drug resistance genes such as tetracycline（tetA, tetC）, quinolones（QnrVC6, QnrVC4）, carbapenems（varG）, and 163 kinds of virulence genes related to flagella（fla E） and iron ion absorption（hutA）. The results of MLST analysis showed that there were 41 ST types(one untyped ), 32 new ST types were generated, and 38 isolates were new ST types, mainly ST326（5.88%，3/51） and ST667（5.88%，3/51）. SNP showed that all strains were divided into three branches, and the branches in the phylogenetic tree were longer, and had higher homologous recombination. Conclusion In Pudong New Area, the contamination of Vibrio vulnificus was serious in shellfish, fish, shrimp, ready-to-eat aquatic products and animal meat. The drug resistance spectrum is wide and there are multiple drug resistance phenomena. It carries a variety of virulence genes and drug-resistant genes, and is highly homologous recombination, which has certain food safety risks, and should be strengthened monitoring and management.
- Full text:202511131708032968115.Pathogenic genetic characteristics of Vibrio vulnificus in commercial foods in Pudong New Area of Shanghai.pdf
